Wang Tongxin, Chen Zhixia, Zhang Wei
Department of Neurosurgery, Yidu Central Hospital of Weifang, Qingzhou, Shandong 262500, P.R. China.
Oncol Lett. 2017 Dec;14(6):7197-7200. doi: 10.3892/ol.2017.7103. Epub 2017 Sep 29.
The objective of this study was to investigate the mechanism of the function of Wnt signaling pathway in regulating autophagy and inflammatory response in glioma cells. Human brain glioma cells U118 were selected and divided into three groups: i) the Wnt signaling inhibitor IWR-1 group (the observation group); ii) the PBS negative control group (the PBS group) and iii) the blank control group. After 24 h culture, Wnt5a/β-catenin protein, autophagy marker, microtubule-associated-proteins-1A1B-light-chain-3C (LC-3) II and Beclin I, and inflammatory factors IL-6 and TNF-α protein expression levels were evaluated using western blotting. Compared with both control groups, Wnt5a/β-catenin, IL-6 and TNF-α protein expression levels were significantly lower, and LC-3II and Beclin I protein expression levels were significantly higher in the observation group. In conclusion, Wnt5a/β-catenin signaling pathway regulates autophagy and inflammatory response of glioma cells.
本研究的目的是探讨Wnt信号通路在调节胶质瘤细胞自噬和炎症反应中的作用机制。选取人脑胶质瘤细胞U118并分为三组：i）Wnt信号抑制剂IWR-1组（观察组）；ii）PBS阴性对照组（PBS组）；iii）空白对照组。培养24小时后，采用蛋白质印迹法评估Wnt5a/β-连环蛋白、自噬标志物微管相关蛋白1A/1B轻链3C（LC-3）II和Beclin I以及炎性因子IL-6和TNF-α蛋白表达水平。与两个对照组相比，观察组中Wnt5a/β-连环蛋白、IL-6和TNF-α蛋白表达水平显著降低，而LC-3II和Beclin I蛋白表达水平显著升高。综上所述，Wnt5a/β-连环蛋白信号通路调节胶质瘤细胞的自噬和炎症反应。
