Section on Neural Developmental Dynamics, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Section on Neural Developmental Dynamics, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Development. 2018 Jul 30;145(14):dev162453. doi: 10.1242/dev.162453.
The zebrafish posterior lateral line primordium migrates along a path defined by the chemokine Cxcl12a, periodically depositing neuromasts, to pioneer formation of the zebrafish posterior lateral line system. , known for its role in promoting cell migration, is expressed in leading cells of the primordium in response to Cxcl12a, whereas its expression in trailing cells is inhibited by Fgf signaling. knockdown delays initiation of primordium migration. This delay is associated with aberrant expansion of () and reduction of expression in the leading part of the primordium. Co-injection of morpholino with mRNA prevents the initial delay in migration and restores normal expression of and The delay in initiating primordium migration in morphants is accompanied by a delay in sequential formation of trailing Fgf signaling centers and associated protoneuromasts. This delay is not specifically associated with knockdown of but also with other manipulations that delay migration of the primordium. These observations reveal an unexpected link between the initiation of collective migration and sequential formation of protoneuromasts in the primordium.
斑马鱼后侧线原基沿 Cxcl12a 定义的路径迁移，周期性地沉积神经丘，从而开拓斑马鱼后侧线系统的形成。已知其在促进细胞迁移中起作用，在原基的前导细胞中响应 Cxcl12a 表达，而在后导细胞中的表达受 Fgf 信号抑制。 敲低会延迟原基迁移的开始。这种延迟与原基前导部分 () 的异常扩张和 的表达减少有关。与 mRNA 共注射 可以防止最初的迁移延迟，并恢复原基前导部分的 和 的正常表达。 在 形态发生素中，原基迁移的起始延迟伴随着尾随 Fgf 信号中心和相关原始神经丘的形成的顺序延迟。这种延迟不仅与 的敲低有关，而且与其他延迟原基迁移的操作有关。这些观察结果揭示了集体迁移的起始和原基中原始神经丘的顺序形成之间的意外联系。
