还原型牛铜锌超氧化物歧化酶的制备
Preparation of reduced bovine Cu,Zn superoxide dismutase.
作者信息
Viglino P, Scarpa M, Cocco D, Rigo A
出版信息
Biochem J. 1985 Jul 1;229(1):87-90. doi: 10.1042/bj2290087.
Abstract
N.m.r. and e.p.r. were used to measure the oxidation state of copper in Cu,Zn superoxide dismutase treated with reducing agents such as NaBH4, K4Fe(CN)6, Na2S2O4 and H2O2. The activity and the electrophoretic pattern of the treated enzyme were also studied. On the basis of the reducing ability and of the absence of inactivating effects, NaBH4 was the most suitable reducer of those tested. Some characteristics of the reduction of superoxide dismutase by NaBH4 were further investigated. The results obtained indicate that NaBH4 can be used to prepare, in a few minutes, solutions of completely reduced enzyme without any apparent change of the activity and of the structure.
摘要
利用核磁共振（N.m.r.）和电子顺磁共振（e.p.r.）来测定用诸如硼氢化钠（NaBH4）、亚铁氰化钾（K4Fe(CN)6）、连二亚硫酸钠（Na2S2O4）和过氧化氢（H2O2）等还原剂处理后的铜锌超氧化物歧化酶中铜的氧化态。还研究了处理后酶的活性和电泳图谱。基于其还原能力以及不存在失活作用，硼氢化钠是所测试的还原剂中最合适的。进一步研究了硼氢化钠还原超氧化物歧化酶的一些特性。所获得的结果表明，硼氢化钠可用于在几分钟内制备完全还原的酶溶液，而酶的活性和结构没有任何明显变化。
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