Mukrimin Mukrimin, Kovalchuk Andriy, Neves Leandro G, Jaber Emad H A, Haapanen Matti, Kirst Matias, Asiegbu Fred O
Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
Department of Forestry, Faculty of Forestry, Hasanuddin University, Makassar, Indonesia.
Front Plant Sci. 2018 Jun 12;9:793. doi: 10.3389/fpls.2018.00793. eCollection 2018.
Root and butt rot caused by members of the species complex is the most economically important disease of conifer trees in boreal forests. Wood decay in the infected trees dramatically decreases their value and causes considerable losses to forest owners. Trees vary in their susceptibility to infection, but the genetic determinants underlying the variation in the susceptibility are not well-understood. We performed the identification of Norway spruce genes associated with the resistance to infection using genome-wide exon-capture approach. Sixty-four clonal Norway spruce lines were phenotyped, and their responses to inoculation were determined by lesion length measurements. Afterwards, the spruce lines were genotyped by targeted resequencing and identification of genetic variants (SNPs). Genome-wide association analysis identified 10 SNPs located within 8 genes as significantly associated with the larger necrotic lesions in response to inoculation. The genetic variants identified in our analysis are potential marker candidates for future screening programs aiming at the differentiation of disease-susceptible and resistant trees.
由该物种复合体成员引起的根腐病和干基腐朽病是北方森林中针叶树最重要的经济病害。受感染树木的木材腐朽会大幅降低其价值，给森林所有者造成巨大损失。树木对感染的易感性各不相同，但对这种易感性差异背后的遗传决定因素却了解甚少。我们采用全基因组外显子捕获方法鉴定了与挪威云杉抗感染相关的基因。对64个挪威云杉无性系进行了表型分析，并通过测量病斑长度来确定它们对接种的反应。之后，通过靶向重测序和遗传变异（单核苷酸多态性，SNPs）鉴定对云杉无性系进行基因分型。全基因组关联分析确定了位于8个基因内的10个单核苷酸多态性与接种后较大的坏死病斑显著相关。我们分析中鉴定出的遗传变异是未来旨在区分感病和抗病树木的筛选计划的潜在标记候选物。
