Varela I, Mérida I, Villalba M, Vivanco F, Mato J M
Biochem Biophys Res Commun. 1985 Aug 30;131(1):477-83. doi: 10.1016/0006-291x(85)91827-3.
We have obtained a rabbit antiserum that specifically immunoprecipitates the 50K and 25K proteins of rat liver phospholipid methyltransferase. Exposure of intact rat hepatocytes preincubated with [32P]phosphate to glucagon induces a time-dependent phosphorylation of the 50K protein of phospholipid methyltransferase. The incorporation of 32P into the 50K protein was only on phosphoserine. These data support the concept that the activation of rat liver phospholipid methyltransferase by glucagon is mediated by phosphorylation of the enzyme.
我们获得了一种兔抗血清，它能特异性免疫沉淀大鼠肝脏磷脂甲基转移酶的50K和25K蛋白。用[32P]磷酸盐预孵育的完整大鼠肝细胞暴露于胰高血糖素会诱导磷脂甲基转移酶50K蛋白发生时间依赖性磷酸化。32P掺入50K蛋白仅在磷酸丝氨酸上。这些数据支持这样的概念，即胰高血糖素对大鼠肝脏磷脂甲基转移酶的激活是由该酶的磷酸化介导的。
