de Albuquerque Pedro Paulo Feitosa, de Melo Renata Pimentel Bandeira, de Farias Brito Marilene, Bovino Fernanda, de Souza Mariana Assunção, Lima Anna Monteiro Correia, de Oliveira Emerson Antônio Araújo, de Moraes Pereira Helder, Mota Rinaldo Aparecido
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, PE, Brazil.
Department of Epidemiology and Public Health, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Trop Anim Health Prod. 2018 Dec;50(8):1929-1935. doi: 10.1007/s11250-018-1650-3. Epub 2018 Jun 26.
Paratuberculosis is an incurable disease in ruminants with great worldwide economic impact, caused by Mycobacterium avium subsp. paratuberculosis (MAP). The objective of this study was to carry out a study of the molecular epidemiology of the MAP using the restriction enzyme analysis (REA) technique of IS1311 MAP region in biological samples of feces, intestinal tissue, and mesenteric lymph nodes of cattle and buffaloes from six Brazilian states. In total, 109 samples of feces and tissues of cattle and buffaloes were collected from animal paratuberculosis suspected. Twenty-five samples were positive in the detection of the DNA of the IS900 region of MAP and it was possible to type 18 strains in the analysis of the region IS1311, being 100% of them identified as belonging to subtype Bison MAP strain. This is the first epidemiological molecular study of MAP in Brazil. The results indicate that paratuberculosis is widespread in cattle and in buffaloes in several regions of Brazil, and the subtype Bison MAP strain was the only one identified in the samples analyzed in this study, demonstrating the similarity between the strains from different states tested. These results provide the necessary support for the implementation of paratuberculosis control strategies in cattle and buffaloes in Brazil.
副结核病是一种反刍动物的不治之症，在全球范围内造成巨大经济影响，由鸟分枝杆菌副结核亚种（MAP）引起。本研究的目的是利用IS1311 MAP区域的限制性内切酶分析（REA）技术，对来自巴西六个州的牛和水牛的粪便、肠道组织和肠系膜淋巴结等生物样本中的MAP进行分子流行病学研究。总共从疑似患有动物副结核病的牛和水牛身上采集了109份粪便和组织样本。在MAP的IS900区域DNA检测中，有25个样本呈阳性，并且在IS1311区域分析中能够对18株菌株进行分型，其中100%被鉴定为属于野牛MAP菌株亚型。这是巴西首次对MAP进行的流行病学分子研究。结果表明，副结核病在巴西多个地区的牛和水牛中广泛存在，并且在本研究分析的样本中，野牛MAP菌株亚型是唯一鉴定出的亚型，这表明来自不同测试州的菌株具有相似性。这些结果为巴西实施牛和水牛副结核病控制策略提供了必要支持。
