Clinics Department, State University of Maranhão, Cidade Universitária Paulo VI, Tirirical, P.O. BOX 09, São Luís, MA, 65055-970, Brazil.
Department of Pathology, State University of Maranhão, São Luís, MA, Brazil.
Braz J Microbiol. 2020 Sep;51(3):1383-1390. doi: 10.1007/s42770-020-00267-4. Epub 2020 Apr 14.
Paratuberculosis is an infectious, chronic, and incurable disease that affects ruminants, causing enteritis and chronic granulomatous lymphadenitis, characterized by malabsorption syndrome, its agent is the Mycobacterium avium subsp. paratuberculosis (MAP). Thus, the objective of this work was to identify and characterize MAP in buffalo herds slaughtered in Baixada Maranhense region. Samples of intestines, mesenteric lymph nodes, and ileocecal valves were collected from 115 buffaloes slaughtered at Baixada Maranhense slaughterhouses to perform the diagnosis by histopathological examination using staining with Hematoxylin and Eosin (H&E) and Ziehl-Neelsen, bacterial isolation, and real-time PCR. In the histopathology by H&E staining, there was evidence suggestive of paratuberculosis in 30% (31/115) of the buffaloes. With Ziehl-Neelsen staining, acid-fast bacilli (AFB) were visualized in 27% (26/115) of the tissue samples analyzed. MAP was isolated in 4.3% (5/115) of the fecal samples subjected to bacterial culture. The samples inoculated in HEYM with mycobactin J produced colonies identified with MAP according to their own morphological characteristics such as round, white, smooth and slightly rough, alcohol-acid staining, and slow growth with 8 weeks of incubation and mycobactin dependence. The agent confirmation was performed in five bacterial isolates (4.3%) and 15 (13%) fragments of jejunum, ileum, and mesenteric lymph node by the IS900 real-time PCR technique. The results of the present study demonstrate the subclinical occurrence of paratuberculosis in flocks of buffalo slaughtered in slaughterhouses of Baixada Maranhense.
副结核病是一种感染性、慢性和无法治愈的疾病，影响反刍动物，引起肠炎和慢性肉芽肿性淋巴结炎，其特征是吸收不良综合征，其病原体是鸟分枝杆菌副结核亚种（MAP）。因此，本工作的目的是鉴定和表征在巴伊亚马兰亨塞地区屠宰的水牛群中的 MAP。从巴伊亚马兰亨塞屠宰场屠宰的 115 头水牛中采集肠道、肠系膜淋巴结和回盲瓣样本，通过苏木精和曙红（H&E）染色和齐尔-尼尔森染色进行组织病理学检查、细菌分离和实时 PCR 进行诊断。在 H&E 染色的组织病理学检查中，有 30%（31/115）的水牛有副结核病的证据。用齐尔-尼尔森染色，在分析的 27%（26/115）的组织样本中可见抗酸杆菌（AFB）。在接受细菌培养的 4.3%（5/115）的粪便样本中分离出 MAP。在 HEYM 中接种的样本用分枝菌酸 J 产生了根据其自身形态特征（如圆形、白色、光滑略粗糙、酒精酸染色和 8 周孵育时生长缓慢且依赖分枝菌酸）识别为 MAP 的菌落。通过 IS900 实时 PCR 技术，在 5 个细菌分离物（4.3%）和 15 个（13%）空肠、回肠和肠系膜淋巴结片段中确认了该病原体。本研究的结果表明，在巴伊亚马兰亨塞屠宰场屠宰的水牛群中存在副结核病的亚临床发生。
