Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobelstr. 12, 70569, Stuttgart, Germany.
Max Planck Institute for Developmental Biology, Max-Planck-Ring 5, 72076, Tübingen, Germany.
Macromol Biosci. 2018 Sep;18(9):e1800104. doi: 10.1002/mabi.201800104. Epub 2018 Jun 26.
Light-induced release systems can be triggered remotely and are of interest for many controlled release applications due to the possibility for spatio-temporal release control. In this study a biotin-functionalized photocleavable macromer is incorporated with an o-nitrobenzyl moiety into gelatin methacryloyl(-acetyl) hydrogels via radical cross-linking. Stronger immobilization of streptavidin-coupled horseradish peroxidase occurs in linker-functionalized hydrogels compared to pure gelatin methacryloyl(-acetyl) hydrogels, and a controlled release of the streptavidin conjugate upon UV-irradiation is possible. Liquid chromatography coupled to mass spectrometry (LC-MS) analysis of aqueous linker solutions allows the identification of the main cleavage products and the cleavage kinetics. Thus, it is shown that a significant hydrolysis of the linker occurs at 37 °C. Nevertheless the system reported here is a promising controlled release scaffold for proteins and application in tissue engineering, if background releases of the immobilized drug are tolerable.
光诱导释放系统可以远程触发，由于具有时空释放控制的可能性，因此引起了许多控制释放应用的兴趣。在这项研究中，通过自由基交联将生物素功能化的光可裂解大分子单体与邻硝基苄基部分结合到明胶甲基丙烯酰（-乙酰基）水凝胶中。与纯明胶甲基丙烯酰（-乙酰基）水凝胶相比，在连接子功能化水凝胶中固定化链霉亲和素偶联辣根过氧化物酶的能力更强，并且可以在 UV 照射下进行链霉亲和素缀合物的受控释放。通过与质谱联用的液相色谱（LC-MS）分析水合连接子溶液，可以鉴定主要的裂解产物和裂解动力学。因此，表明在 37°C 时连接子发生了明显的水解。但是，如果可以容忍固定药物的背景释放，则此处报道的系统是一种有前途的蛋白质控制释放支架，可用于组织工程。
