Nuanpeng Sunan, Thanonkeo Sudarat, Klanrit Preekamol, Thanonkeo Pornthap
Khon Kaen University, Faculty of Technology, Department of Biotechnology, Khon Kaen, Thailand.
Mahasarakham University, Walai Rukhavej Botanical Research Institute, Mahasarakham, Thailand.
Braz J Microbiol. 2018 Nov;49 Suppl 1(Suppl 1):140-150. doi: 10.1016/j.bjm.2017.12.011. Epub 2018 Mar 13.
Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20×20×5mm is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined. The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively. The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.
利用固定在藻酸盐-丝瓜络基质（ALM）中的耐热酿酒酵母菌株DBKKUY-53，成功开发了从甜高粱汁（SSJ）生产乙醇的方法。如本研究中所发现的，尺寸为20×20×5mm的ALM因其紧凑的结构和长期稳定性而对细胞固定有效。ALM固定化细胞系统比游离悬浮细胞系统表现出更高的乙醇生产效率。通过使用中心复合设计（CCD），确定了ALM固定化细胞从SSJ生产乙醇的最佳条件。在最佳条件下，使用ALM固定化细胞获得的最大乙醇浓度和乙醇体积产率分别为97.54g/L和1.36g/Lh。使用ALM固定化细胞至少连续六个批次（360小时）成功，且乙醇生产效率没有任何损失，表明它们在工业乙醇生产中的潜在应用。
