酿酒酵母DBKKUY - 53固定在海藻酸钠 - 丝瓜络基质上从甜高粱生产乙醇。
Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices.
作者信息
Nuanpeng Sunan, Thanonkeo Sudarat, Klanrit Preekamol, Thanonkeo Pornthap
机构信息
Khon Kaen University, Faculty of Technology, Department of Biotechnology, Khon Kaen, Thailand.
Mahasarakham University, Walai Rukhavej Botanical Research Institute, Mahasarakham, Thailand.
出版信息
Braz J Microbiol. 2018 Nov;49 Suppl 1(Suppl 1):140-150. doi: 10.1016/j.bjm.2017.12.011. Epub 2018 Mar 13.
Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20×20×5mm is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined. The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively. The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.
利用固定在藻酸盐-丝瓜络基质（ALM）中的耐热酿酒酵母菌株DBKKUY-53，成功开发了从甜高粱汁（SSJ）生产乙醇的方法。如本研究中所发现的，尺寸为20×20×5mm的ALM因其紧凑的结构和长期稳定性而对细胞固定有效。ALM固定化细胞系统比游离悬浮细胞系统表现出更高的乙醇生产效率。通过使用中心复合设计（CCD），确定了ALM固定化细胞从SSJ生产乙醇的最佳条件。在最佳条件下，使用ALM固定化细胞获得的最大乙醇浓度和乙醇体积产率分别为97.54g/L和1.36g/Lh。使用ALM固定化细胞至少连续六个批次（360小时）成功，且乙醇生产效率没有任何损失，表明它们在工业乙醇生产中的潜在应用。
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