ICFO-Institut de Ciéncies Fotòniques , The Barcelona Institute of Science and Technology , 08860 Castelldefels, Barcelona , Spain.
ICREA-Institució Catalana de Recerca i Estudis Avançats , 08010 Barcelona , Spain.
ACS Sens. 2018 Jul 27;3(7):1376-1384. doi: 10.1021/acssensors.8b00305. Epub 2018 Jul 11.
The need for point-of-care devices able to detect diseases early and monitor their status, out of a lab environment, has stimulated the development of compact biosensing configurations. Whereas localized surface plasmon resonance (LSPR) sensing integrated into a state-of-the-art microfluidic chip stands as a promising approach to meet this demand, its implementation into an operating sensing platform capable of quantitatively detecting a set of molecular biomarkers in an unknown biological sample is only in its infancy. Here, we present an on-chip LSPR sensor capable of performing automatic, quantitative, and multiplexed screening of biomarkers. We demonstrate its versatility by programming it to detect and quantify in human serum four relevant human serum protein markers associated with breast cancer.
对能够在实验室环境之外及早检测疾病并监测其状况的即时检测设备的需求，刺激了紧凑型生物传感配置的发展。局部表面等离子体共振（LSPR）感测与最先进的微流控芯片集成，是满足这一需求的很有前途的方法，但是将其实现为能够在未知生物样本中定量检测一组分子生物标志物的运行传感平台，仍处于起步阶段。在这里，我们提出了一种能够进行自动、定量和多重生物标志物筛选的芯片上 LSPR 传感器。我们通过编程使其检测和量化与乳腺癌相关的人血清中的四个相关人血清蛋白标志物，展示了其多功能性。
