ETH Zurich, Department of Biosystems Science and Engineering, Mattenstrasse 26, Basel, 4058, Switzerland.
Analyst. 2013 Mar 7;138(5):1450-8. doi: 10.1039/c3an36616b.
Nanohole array-based biosensors integrated with a microfluidic concentration gradient generator were used for imaging detection and quantification of ovarian cancer markers. Calibration curves based on controlled concentrations of the analyte were created using a microfluidic stepped diffusive mixing scheme. Quantification of samples with unknown concentration of analyte was achieved by image-intensity comparison with the calibration curves. The biosensors were first used to detect the immobilization of ovarian cancer marker antibodies, and subsequently applied for the quantification of the ovarian cancer marker r-PAX8 (with a limit of detection of about 5 nM and a dynamic range from 0.25 to 9.0 μg.mL(-1)). The proposed biosensor demonstrated the ability of self-generating calibration curves on-chip in an integrated microfluidic platform, representing a further step towards the development of comprehensive lab-on-chip biomedical diagnostics based on nanohole array technology.
基于纳米孔阵列的生物传感器与微流控浓度梯度发生器集成，用于卵巢癌标志物的成像检测和定量。使用微流控阶跃扩散混合方案，基于分析物的受控浓度创建校准曲线。通过与校准曲线的图像强度比较，实现了对未知浓度分析物样品的定量。生物传感器首先用于检测卵巢癌标志物抗体的固定化，随后用于定量卵巢癌标志物 r-PAX8（检测限约为 5 nM，动态范围为 0.25 至 9.0 μg.mL(-1)）。所提出的生物传感器在集成微流控平台上展示了在芯片上自我生成校准曲线的能力，这是朝着基于纳米孔阵列技术开发全面的芯片实验室生物医学诊断迈出的进一步一步。
