Brown Judy, Crivello Julianna, O'Neill Rachel J
Department of Allied Health Sciences and Institute for Systems Genomics, University of Connecticut, Storrs, CT, 06269, USA.
Department of Molecular and Cell Biology and Institute for Systems Genomics, University of Connecticut, Storrs, CT, 06269-1131, USA.
Mamm Genome. 2018 Jun;29(5-6):344-352. doi: 10.1007/s00335-018-9754-7. Epub 2018 Jun 15.
Species across the rodent genus Peromyscus have become prominent models for studying diverse mechanistic and evolutionary processes, including chromosome evolution, infectious disease transmission and human health, ecological adaptation, coat color variation, and parental care. Supporting such diverse research programs has been the development of genetic and genomic resources for species within this genus, including genome data, interspecific chromosome homologies, and a recently developed genetic map. Based on interspecific hybrids between the deer mouse (Peromyscus maniculatus bairdii) and the old-field, or beach, mouse (Peromyscus polionotus) and backcross progeny to Peromyscus maniculatus, a linkage map was developed based on 190 genes and 141 microsatellite loci. However, resolution of several linkage groups with respect to chromosome assignment was lacking and four chromosomes (8, 16, 20, and 21) were not clearly delineated with linkage data alone. The recent development of a high-density map for Peromyscus proved ineffective in resolving chromosome linkage for these four chromosomes. Herein we present an updated linkage map for Peromyscus maniculatus, including linkage group-chromosome assignments, using fluorescence in situ hybridization mapping of BACs and whole chromosome paints. We resolve the previously conflicting chromosome assignment of linkage groups to Chromosomes 8, 16, 20, and 21, and confirm the assignment of linkage groups to Chromosomes 18 and 22. This updated linkage map with validated chromosome assignment provides a solid foundation for chromosome nomenclature for this species.
鹿鼠属（Peromyscus）的各个物种已成为研究多种机制和进化过程的重要模型，这些过程包括染色体进化、传染病传播与人类健康、生态适应、毛色变异以及亲代抚育。该属内物种的遗传和基因组资源的开发，包括基因组数据、种间染色体同源性以及最近开发的遗传图谱，为这些多样的研究项目提供了支持。基于鹿鼠（Peromyscus maniculatus bairdii）与旧域鼠或海滩鼠（Peromyscus polionotus）之间的种间杂交以及与鹿鼠的回交后代，构建了一个基于190个基因和141个微卫星位点的连锁图谱。然而，几个连锁群在染色体定位方面缺乏分辨率，仅靠连锁数据无法清晰界定四条染色体（8号、16号、20号和21号）。最近为鹿鼠属开发的高密度图谱在解析这四条染色体的连锁关系方面效果不佳。在此，我们展示了一个更新后的鹿鼠（Peromyscus maniculatus）连锁图谱，包括使用BACs荧光原位杂交图谱和全染色体涂染进行的连锁群 - 染色体定位。我们解决了之前连锁群与8号、16号、20号和21号染色体之间相互矛盾的染色体定位问题，并确认了连锁群与18号和22号染色体的定位。这个具有经过验证的染色体定位的更新连锁图谱为该物种的染色体命名提供了坚实基础。
