The Key Laboratory of Animal Vaccine and Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Arch Virol. 2018 Oct;163(10):2841-2847. doi: 10.1007/s00705-018-3914-7. Epub 2018 Jun 14.
A newly emerging porcine circovirus, designated PCV3, has been reported in various countries (USA, Poland, South Korea and China) since 2017. Its presence may be associated with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, and multisystem inflammation. In this study, we report identification of PCV3 in cases of reproductive failure in various regions in Hunan, China. From January 2015 to December 2016, sera were collected from 190 sows from seven farms with reproductive problems. Specifically, 85 samples were from sows with a history of reproductive failure, whereas the remaining 105 were from healthy sows. The PCV3-positive rate was significantly higher in sows with reproductive failure (45.9%) than in healthy sows (21.9%), based on quantitative PCR (qPCR) assays. Although phylogenetic analysis based on the cap gene suggested that these PCV3 isolates belonged to the clade PCV3a, amino acid sequence variations in the Cap protein still occurred among these isolates, and these might have contributed to antigenic alterations of the Cap protein, based on the Jameson-Wolf antigenic index. Finally, we concluded that PCV3 was circulating in sows in Hunan province, China. However, the association of PCV3 with reproductive failure in sows and its potential for vertical transmission need to be studied further.
自 2017 年以来，一种新出现的猪圆环病毒，称为 PCV3，已在多个国家（美国、波兰、韩国和中国）被报道。其存在可能与猪皮炎肾病综合征（PDNS）、繁殖失败和多系统炎症有关。在本研究中，我们报告了在中国湖南不同地区繁殖失败病例中 PCV3 的鉴定。2015 年 1 月至 2016 年 12 月，从 7 个农场的 190 头有繁殖问题的母猪中采集血清。具体来说，85 份样本来自有繁殖失败史的母猪，而其余 105 份来自健康母猪。基于定量 PCR（qPCR）检测，繁殖失败的母猪中 PCV3 的阳性率（45.9%）明显高于健康母猪（21.9%）。尽管基于衣壳基因的系统进化分析表明，这些 PCV3 分离株属于 PCV3a 分支，但在这些分离株中 Cap 蛋白的氨基酸序列变异仍然存在，这可能导致 Cap 蛋白的抗原性改变，基于 Jameson-Wolf 抗原指数。最后，我们得出结论，PCV3 在中国湖南省的母猪中流行。然而，PCV3 与母猪繁殖失败的关系及其垂直传播的潜力仍需进一步研究。
