Yue Weidong, Li Yilei, Zhang Xinrong, He Junping, Ma Haili
College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.
Front Vet Sci. 2022 May 23;9:820914. doi: 10.3389/fvets.2022.820914. eCollection 2022.
Porcine circovirus disease is currently the greatest threat to pig farming. Four main porcine circovirus genotypes are circulating worldwide.
The study aimed to assess the prevalence of porcine circovirus genotypes in the central part of Shanxi province.
We investigated the prevalence of porcine circovirus type 2 (PCV2), porcine circovirus type 3 (PCV3), and porcine circovirus type 4 (PCV4). Porcine circoviruses were analyzed by polymerase chain reaction (PCR) in the lung tissues of 180 pigs from 7 slaughterhouses in central Shanxi, China.
The prevalence of PCV2, PCV3, and PCV4 were 56.8, 80, and 9.4%, respectively, and the negative rate was 10% for all three pathogens. The co-infection with PCV2 + PCV3, PCV2 + PCV4, PCV3 + PCV4, and PCV2 + PCV3 + PCV4 were 47.2, 7.4, 7.4, and 5.6%, respectively. Among PCV4-positive samples, the positive rate of PCV4 + PCV2 was 52.9% (9/17), whereas that of PCV4 + PCV3 was 100% (17/17). On the other hand, PCV2 and PCV3 were detected in 57.1% (93/163) and in 78.5% (128/163) of PCV4-negative samples, respectively. Phylogenetic analysis demonstrated that PCV2, PCV3, and PCV4 were not in the same clade and were distant from each other.
The high positive rates of PCV3, PCV2 + PCV3, and PCV3 + PCV4 suggest that PCV3 may play a decisive role in PCV2 and PCV4 infections. Therefore, further control of PCV3 is needed to reduce the spread of the virus.
猪圆环病毒病是目前养猪业面临的最大威胁。四种主要的猪圆环病毒基因型在全球范围内传播。
本研究旨在评估山西省中部地区猪圆环病毒基因型的流行情况。
我们调查了猪圆环病毒2型（PCV2）、猪圆环病毒3型（PCV3）和猪圆环病毒4型（PCV4）的流行情况。采用聚合酶链反应（PCR）对来自中国山西省中部7家屠宰场的180头猪的肺组织中的猪圆环病毒进行分析。
PCV2、PCV3和PCV4的流行率分别为56.8%、80%和9.4%，三种病原体的阴性率均为10%。PCV2 + PCV3、PCV2 + PCV4、PCV3 + PCV4和PCV2 + PCV3 + PCV4的共同感染率分别为47.2%、7.4%、7.4%和5.6%。在PCV4阳性样本中，PCV4 + PCV2的阳性率为52.9%（9/17），而PCV4 + PCV3的阳性率为100%（17/17）。另一方面，在PCV4阴性样本中，分别有57.1%（93/163）和78.5%（128/163）检测到PCV2和PCV3。系统发育分析表明，PCV2、PCV3和PCV4不在同一进化枝中，彼此距离较远。
PCV3、PCV2 + PCV3和PCV3 + PCV4的高阳性率表明PCV3可能在PCV2和PCV4感染中起决定性作用。因此，需要进一步控制PCV3以减少病毒传播。
