Pon D J, Stewart D J, Sen A K
Can J Biochem Cell Biol. 1985 Jul;63(7):774-7. doi: 10.1139/o85-097.
A low molecular weight substance is present in the soluble fraction of the rat submandibular gland which activates Na+,K+-ATPase. Varying amounts of cytosolic protein from rat submandibular gland were added to a heavy microsomal preparation of the same tissue. A dose-dependent activation of the Na+,K+-ATPase was observed, with a peak activation of approximately 82% occurring when 9.0 micrograms/mL of cytosolic protein was included in the assay. The activating factor is heat stable and soluble following heat treatment. The factor elutes at a molecular mass of 600 daltons as determined by molecular sieve column chromatography. Ultraviolet-visible scanning of the elute material results in an absorbance at 210 nm, which is characteristic of a low molecular weight peptide.
大鼠下颌下腺的可溶性部分存在一种能激活钠钾ATP酶的低分子量物质。将不同量的大鼠下颌下腺胞质蛋白添加到同一组织的重微粒体制剂中。观察到钠钾ATP酶呈剂量依赖性激活，当测定中包含9.0微克/毫升的胞质蛋白时，激活峰值约为82%。激活因子热稳定，热处理后可溶。通过分子筛柱色谱法测定，该因子在分子量为600道尔顿时洗脱。对洗脱物质进行紫外可见扫描，在210纳米处有吸光度，这是低分子量肽的特征。
