Foulks J G, Morishita L
Can J Physiol Pharmacol. 1985 Jun;63(6):693-703. doi: 10.1139/y85-115.
D2O and perchlorate manifest opposing effects on the contractile function of skeletal muscle (amplitude of twitches and maximum K contractures, potential dependence of contraction activation and inactivation), and when combined the influence of one may effectively antagonize that of the other. The ratio of perchlorate concentrations required to produce effects of equal intensity (e.g., twitch enhancement and restoration of maximum K contractures in media lacking divalent cations or containing a depressant concentration of a cationic amphipath) in H2O and D2O solutions was generally rather constant. These findings are compatible with the view that both agents can influence contractile function by virtue of their effects on solvent structure. In the absence of divalent cations, the effects of reduced temperature resemble those of D2O whereas the effects of increased temperature resemble those of the chaotropic anion. However, in other media, variation in temperature was found to result in additional nonsolvent effects so that low temperature could oppose rather than enhance the effects of D2O. These observations are discussed in terms of a model postulates a role for solvent influences on the kinetics of two separate potential-dependent conformational transitions of membrane proteins which mediate the activation and inactivation of contraction in skeletal muscle.
重水（D₂O）和高氯酸盐对骨骼肌的收缩功能表现出相反的作用（抽搐幅度和最大钾挛缩、收缩激活和失活的电位依赖性），并且两者联合使用时，其中一种的影响可能会有效拮抗另一种的影响。在H₂O和D₂O溶液中，产生同等强度效应（例如，在缺乏二价阳离子或含有抑制浓度阳离子两亲物的介质中抽搐增强和最大钾挛缩恢复）所需的高氯酸盐浓度之比通常相当恒定。这些发现与以下观点一致，即两种试剂都可以通过其对溶剂结构的影响来影响收缩功能。在没有二价阳离子的情况下，降低温度的影响类似于重水的影响，而升高温度的影响类似于离液序列高的阴离子的影响。然而，在其他介质中，发现温度变化会导致额外的非溶剂效应，因此低温可能会对抗而不是增强重水的影响。这些观察结果根据一个模型进行了讨论，该模型假设溶剂对膜蛋白两个独立的电位依赖性构象转变的动力学有影响，这两个构象转变介导骨骼肌收缩的激活和失活。
