Toritsuka Michihiro, Makinodan Manabu, Yamauchi Takahira, Yamashita Yasunori, Ikawa Daisuke, Komori Takashi, Kimoto Sohei, Hamano-Iwasa Kaori, Matsuzaki Hideo, Kishimoto Toshifumi
Department of Psychiatry, Nara Medical University School of Medicine, Nara, 634-8521, Japan.
Research Center for Child Mental Development, University of Fukui, Fukui, 910-1193, Japan.
In Vitro Cell Dev Biol Anim. 2018 Aug;54(7):523-527. doi: 10.1007/s11626-018-0267-1. Epub 2018 Jun 12.
Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.
淋巴母细胞系（LCLs）是几乎永生化的B淋巴细胞，被用作人类细胞的长期来源，用于基因表达分析研究。然而，关于LCLs细胞特征稳定性的研究很少。为了解决这个问题，我们测量了不同传代次数的LCLs中的基因表达，发现基因表达在10代内发生了显著变化。特别是，甘油醛-3-磷酸脱氢酶（GAPDH）这一著名的管家基因的表达在传代培养过程中变化很大；因此，将GAPDH用作内对照可能不合适。总之，本研究强调了在测定LCLs基因表达时需要谨慎。
