Analytical Chemistry and Chemical Metallurgy Division, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże St. Wyspiańskiego 27, 50-370, Wrocław, Poland.
Veterinary Clinic "Brynów", Brynowska 25c, 40-585, Katowice, Poland.
Biol Trace Elem Res. 2019 Feb;187(2):357-366. doi: 10.1007/s12011-018-1389-8. Epub 2018 Jun 9.
Influence of fixation medium and storage conditions as well as impact of sample mineralisation procedure on determination of minerals in human fascia lata was examined and discussed. Freezing and storage in 10% neutral buffered formalin solution and in 2.5% glutaraldehyde were used as the preservation methods of the samples. The concentrations of, both essential and toxic, elements were measured by ICP-OES method in fascia lata samples mineralised with concentrated nitric acid in a closed microwave system and in open vessels heated on a hot plate. Freezing was found as the best preserving method of fascia lata samples because of the number of elements determined and determination precision. The trace element (Cd, Cr, Cu, Fe, Ni, Sr, Zn) concentrations obtained in samples decomposed using the conventional hot plate were different from analogous measurements in solutions obtained after application of closed vessels and microwave energy assistance. Differences between the mineral compositions of fascia samples variously preserved and mineralised were statistically evaluated and discussed. Interelement correlations were analysed taking into account an impact of various methods of sample conservation. Strong, positive association between element content was discovered for Cr-Ba, Mn-Ba, P-Ba, Sr-Ba, Sr-Ca, Zn-Ca, Mn-Cr, Pb-Cr, Sr-Cr, Mg-Fe, P-Fe, Pb-Ni, Ti-Ni and Sr-P pairs of elements.
研究并讨论了固定介质和储存条件的影响以及样品矿化程序对人体阔筋膜中矿物质测定的影响。将冷冻和储存在 10%中性缓冲福尔马林溶液中和 2.5%戊二醛中作为样品的保存方法。通过电感耦合等离子体 - 发射光谱法（ICP-OES）在使用浓硝酸在密闭微波系统和在热板上加热的敞口容器中矿化的阔筋膜样品中测量了必需和有毒元素的浓度。冷冻被发现是阔筋膜样品的最佳保存方法，因为可以确定的元素数量和测定精度。使用传统热板分解的样品中的微量元素（Cd、Cr、Cu、Fe、Ni、Sr、Zn）浓度与应用密闭容器和微波能量辅助后获得的溶液中的类似测量值不同。对各种保存和矿化阔筋膜样品的矿物质组成进行了统计学评估和讨论。考虑到样品保存方法的影响，分析了元素之间的相关性。Cr-Ba、Mn-Ba、P-Ba、Sr-Ba、Sr-Ca、Zn-Ca、Mn-Cr、Pb-Cr、Sr-Cr、Mg-Fe、P-Fe、Pb-Ni、Ti-Ni 和 Sr-P 对元素之间存在强烈的正相关关系。
