Lee Young Koung, Kim In-Jung
Cold Spring Harbor Laboratory, 1 Bungtown Road,, Cold Spring Harbor, NY, 11724, USA.
Division of Biological Sciences and Institute for Basic Science/Division of Biological Sciences and Research Institute for Glycoscience, Wonkwang University, Iksan, 54538, South Korea.
Genes Genomics. 2018 Oct;40(10):1053-1062. doi: 10.1007/s13258-018-0712-2. Epub 2018 Jun 8.
The LONGIFOLIA1 (LNG1) gene of Arabidopsis regulates leaf shape by polar cell elongation independent of ROTUNDAFOLIA3 (ROT3). To expand our knowledge on the function of this gens in plant systems, Arabidopsis LNG1 (AtLNG1) was introduced both sense and antisense orientation under the control of 35S CaMV promoter into tobacco plants that lack AtLNG1 homolog. Resulting transgenic tobacco plants were analyzed by their phenotype, anatomy and transcript levels. AtLNG1-overexpressing tobacco lines showed increase in the leaf petiole and leaf blade compared with wild type tobacco line. The overexpressors also showed elongated palisade cells as well as epidermal cells in the leaf length direction, but no increase in cell number. Ectopic expression of AtLNG1 in tobacco plants also increased the expression of cell wall modification-related genes, such as NT_XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE9 (NT_XTH9), NT_XTH15 and NT_XTH33, indicating that these genes appear to be target of AtLNG1. As results of molecular and cellular examination, AtLNG1 seemed to have a conserved functional role in shaping leaf morphology in both Arabidopsis and tobacco.
拟南芥的长叶1（LNG1）基因通过极性细胞伸长来调控叶片形状，且不依赖于圆叶3（ROT3）。为了拓展我们对该基因在植物系统中功能的认识，在35S CaMV启动子的控制下，将拟南芥LNG1（AtLNG1）以正义和反义方向导入缺乏AtLNG1同源物的烟草植株中。通过对所得转基因烟草植株的表型、解剖结构和转录水平进行分析。与野生型烟草品系相比，过表达AtLNG1的烟草品系的叶柄和叶片有所增加。过表达植株在叶片长度方向上的栅栏细胞以及表皮细胞也表现出伸长，但细胞数量没有增加。AtLNG1在烟草植株中的异位表达还增加了细胞壁修饰相关基因的表达，如NT_木葡聚糖内转糖基酶/水解酶9（NT_XTH9）、NT_XTH15和NT_XTH33，这表明这些基因似乎是AtLNG1的作用靶点。作为分子和细胞检测的结果，AtLNG1似乎在拟南芥和烟草的叶片形态塑造中具有保守的功能作用。
