拟南芥CAP的过表达导致转基因烟草植株的细胞扩张减少，进而导致器官尺寸减小。
Over-expression of Arabidopsis CAP causes decreased cell expansion leading to organ size reduction in transgenic tobacco plants.
作者信息
Barrero Roberto A, Umeda Masaaki, Yamamura Saburo, Uchimiya Hirofumi
机构信息
Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan.
出版信息
Ann Bot. 2003 Apr;91(5):599-603. doi: 10.1093/aob/mcg056.
Abstract
Cyclase-associated proteins (CAP) are multifunctional proteins involved in Ras-cAMP signalling and regulation of the actin cytoskeleton. It has recently been demonstrated that over-expression of AtCAP1 in transgenic arabidopsis plants causes severe morphological defects owing to loss of actin filaments. To test the generality of the function of AtCAP1 in plants, transgenic tobacco plants over-expressing an arabidopsis CAP (AtCAP1) under the regulation of a glucocorticoid-inducible promoter were produced. Over-expression of AtCAP1 in transgenic tobacco plants led to growth abnormalities, in particular a reduction in the size of leaves. Morphological alterations in leaves were the result of reduced elongation of epidermal and mesophyll cells.
摘要
环化酶相关蛋白（CAP）是参与Ras - cAMP信号传导和肌动蛋白细胞骨架调节的多功能蛋白。最近有研究表明，在转基因拟南芥植物中过表达AtCAP1会由于肌动蛋白丝的缺失而导致严重的形态缺陷。为了测试AtCAP1在植物中功能的普遍性，构建了在糖皮质激素诱导型启动子调控下过表达拟南芥CAP（AtCAP1）的转基因烟草植株。AtCAP1在转基因烟草植株中的过表达导致生长异常，尤其是叶片尺寸减小。叶片的形态改变是表皮细胞和叶肉细胞伸长减少的结果。
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