Department of Obstetrics and Gynecology, Jingzhou Central Hospital/Affiliated Jingzhou Hospital, Tongji Medical College of Huazhong University of Science and Technology, Jingzhou, China.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):3635-3642. doi: 10.26355/eurrev_201806_15240.
We aimed to explore the expression level and biological function of lncRNA PRNCR1 in preeclampsia (PE).
57 PE patients and 57 normal pregnant women were enrolled in this study. The expression level of PRNCR1 in the maternal placenta of PE patients and normal pregnancy was detected by qRT-PCR. The CCK-8 assay was carried out to determine the cell viability after interference and overexpression of PRNCR1 on trophoblasts. We utilized Western blot to examine the protein level of PRNCR1.
Higher systolic blood pressure, diastolic blood pressure and urinary protein levels in PE patients were observed in comparison with those in normal pregnant women, while the neonatal weight in PE group was markedly lower than that in normal pregnant women. LncRNA PRNCR1 was overexpressed in PE patients, which was positively correlated with systolic blood pressure, diastolic blood pressure and urine protein, whereas negatively correlated with fetal birth weight of PE patients. In addition, the expression of PRNCR1 in BeWo trophoblast cells was significantly decreased after the interference with PRNCR1, while the cell viability increased. However, overexpression of PRNCR1 in HTR-8 cells significantly reduced the viability of cells. Expression levels of p-p38 and p-JNK in PE patients were higher than those in normal pregnancy women, and the expression level of p-ERK was decreased, which suggested that PRNCR1 promoted the progression of PE by modulating the MAPK signaling pathway.
PRNCR1 is highly expressed in PE and promotes the progression of PE by modulating the MAPK signaling pathway.
本研究旨在探讨长链非编码 RNA（lncRNA）PRNCR1 在子痫前期（PE）中的表达水平及其生物学功能。
本研究纳入了 57 例 PE 患者和 57 例正常孕妇。通过 qRT-PCR 检测 PE 患者和正常妊娠孕妇母胎盘中 PRNCR1 的表达水平。通过 CCK-8 检测 PRNCR1 干扰和过表达对滋养细胞活力的影响。采用 Western blot 检测 PRNCR1 蛋白水平。
与正常孕妇相比，PE 患者的收缩压、舒张压和尿蛋白水平较高，而新生儿体重明显低于正常孕妇。PE 患者 lncRNA PRNCR1 表达上调，与收缩压、舒张压和尿蛋白呈正相关，与 PE 患者的胎儿出生体重呈负相关。此外，干扰 PRNCR1 后 BeWo 滋养细胞中 PRNCR1 的表达明显降低，细胞活力增加。然而，过表达 PRNCR1 会显著降低 HTR-8 细胞的活力。PE 患者中 p-p38 和 p-JNK 的表达水平高于正常妊娠妇女，而 p-ERK 的表达水平降低，提示 PRNCR1 通过调节 MAPK 信号通路促进 PE 的进展。
PRNCR1 在 PE 中表达上调，通过调节 MAPK 信号通路促进 PE 的进展。
