Department of General Surgery, Ningbo No. 2 Hospital, Ningbo, China.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):3763-3770. doi: 10.26355/eurrev_201806_15258.
Previous studies have shown that BC029135, a long noncoding RNA (lncRNA) with high conservation, was decreased with high fold change in colorectal cancer (CRC) by using microarray assay. In the present work, we investigated the functions of BC029135 in CRC, as well as the potential mechanisms underlying the malignant phenotype caused by loss of BC029135.
Bioinformatics analysis of lncRNA BC029135 was performed to identify the potential function. The cell lines of HCT-116, SW480, SW620, LoVo, SW1116, and HT29 were used in this study. The mRNA expression of BC029135 and β-actinin was measured by Real-Time Polymerase Chain Reaction (RT-PCR). Western blot analysis was used to determine protein expression of β-actinin, MMP-2, cyclin D1, and c-Myc.
Our research showed that the expression of BC029135 in CRC tissues and cell lines was significantly lower than in adjacent normal tissues. In addition, overexpression of lncRNA BC029135 could inhibit the invasion of CRC cells. We also found that lncRNA BC029135 inhibits Wnt/β-catenin signaling in CRC cell lines.
We suggest that up-regulation of BC029135 suppresses CRC invasion and inactivates Wnt/β-catenin signaling. It will provide new insights for the treatment of colorectal cancer and new clues for clinical treatment.
先前的研究表明，通过微阵列分析，BC029135（一种高度保守的长非编码 RNA（lncRNA））在结直肠癌（CRC）中呈高倍数变化减少。在本工作中，我们研究了 BC029135 在 CRC 中的功能，以及由 BC029135 缺失引起恶性表型的潜在机制。
对 lncRNA BC029135 进行生物信息学分析，以确定其潜在功能。本研究使用了 HCT-116、SW480、SW620、LoVo、SW1116 和 HT29 细胞系。通过实时聚合酶链反应（RT-PCR）测量 BC029135 和β-肌动蛋白 mRNA 的表达。Western blot 分析用于确定β-肌动蛋白、MMP-2、细胞周期蛋白 D1 和 c-Myc 的蛋白表达。
我们的研究表明，CRC 组织和细胞系中 BC029135 的表达明显低于相邻正常组织。此外，lncRNA BC029135 的过表达可抑制 CRC 细胞的侵袭。我们还发现 lncRNA BC029135 在 CRC 细胞系中抑制 Wnt/β-catenin 信号通路。
我们认为上调 BC029135 可抑制 CRC 侵袭并使 Wnt/β-catenin 信号失活。它将为结直肠癌的治疗提供新的见解，并为临床治疗提供新的线索。
