Xu Meng, Guo Xu, Wang Rong-Di, Zhang Zhi-Hang, Jia Yi-Mo, Sun Xu
Department of Colorectal Surgery, Dalian Municipal Central Hospital Affiliated of Dalian Medical University.
Dalian Medical University, Dalian City, Liaoning Province, China.
Medicine (Baltimore). 2020 Feb;99(7):e19066. doi: 10.1097/MD.0000000000019066.
Previous work suggests that the long noncoding RNA HCC associated long non-coding RNA (HANR) is associated with hepatocellular carcinoma (HCC) progression, but its significance in the context of colorectal cancer (CRC) remains to be determined. Therefore, in this study we assessed the prognostic and diagnostic value of HANR in patients suffering from CRC.The HANR expression in 165 pairs of CRC cancer and adjacent non-cancerous prostate tissues was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. Student t test was conducted for intergroup comparison. Pearson correlation test was used for correlation analysis. Survival curves were carried out by the Kaplan-Meier method and evaluated using the log-rank test. Multivariable Cox proportional hazard risk regression model was performed to screen the independent factor affected the prognosis of CRC patients.In this study, levels of HANR were significantly higher in CRC tumor samples relative to adjacent normal tissue samples (P < .001). A ROC analysis suggested HANR expression could be reliably used to differentiate between normal and CRC tumor tissue. In addition, elevated HANR expression was positively correlated with more advanced and aggressive CRC features, such as a larger tumor size (P = .003), increased invasion depth (P = .012), and more advanced TNM stage (P = .011). Survival analyses revealed that elevated HANR expression was correlated with worse overall survival (P = .002) and disease-free survival (P = .003). A multivariate analysis further confirmed the relevance of HANR as an independent predictor of CRC patient outcomes.In summary, these results indicate that the lncRNA HANR is a promising prognostic indicator in CRC patients.
先前的研究表明，长链非编码RNA肝癌相关长链非编码RNA（HANR）与肝细胞癌（HCC）进展相关，但其在结直肠癌（CRC）中的意义仍有待确定。因此，在本研究中，我们评估了HANR在CRC患者中的预后和诊断价值。通过定量逆转录聚合酶链反应（qRT-PCR）分析测量了165对CRC癌组织和相邻非癌前列腺组织中的HANR表达。采用学生t检验进行组间比较。采用Pearson相关检验进行相关性分析。采用Kaplan-Meier法绘制生存曲线，并使用对数秩检验进行评估。采用多变量Cox比例风险回归模型筛选影响CRC患者预后的独立因素。在本研究中，CRC肿瘤样本中HANR水平相对于相邻正常组织样本显著更高（P<0.001）。ROC分析表明，HANR表达可可靠地用于区分正常组织和CRC肿瘤组织。此外，HANR表达升高与更晚期和侵袭性更强的CRC特征呈正相关，如肿瘤更大（P=0.003）、浸润深度增加（P=0.012）和TNM分期更晚（P=0.011）。生存分析显示，HANR表达升高与总生存期较差（P=0.002）和无病生存期较差（P=0.003）相关。多变量分析进一步证实了HANR作为CRC患者预后独立预测指标的相关性。总之，这些结果表明lncRNA HANR是CRC患者中一个有前景的预后指标。
