Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital, Beijing, China.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):3812-3818. doi: 10.26355/eurrev_201806_15265.
To investigate the effect of miR-524 on the proliferation of thyroid cancer and its underlying mechanism.
MiR-524 expression levels in thyroid cancer samples and para-cancer tissues were tested by quantitative Real-time polymerase chain reaction (qRT-PCR). Cells proliferative ability and apoptosis were evaluated through methyl thiazolyl tetrazolium (MTT) and apoptosis assays, respectively. Luciferase reporter assay was used to confirm the regulatory mechanism.
MiR-524 expression was reduced in thyroid cancer specimen (p<0.05). Up-regulated miR-524 expression inhibited the proliferative ability and enhanced cell apoptosis of thyroid cancer cells. SPAG9 was a target gene of miR-524, and was reversely regulated by miR-524.
MiR-524 represses thyroid cancer cell proliferation and induces cell apoptosis via targeting SPAG9.
探讨 miR-524 对甲状腺癌细胞增殖的影响及其作用机制。
采用实时定量聚合酶链反应(qRT-PCR)检测甲状腺癌组织及癌旁组织中 miR-524 的表达水平。通过甲基噻唑基四唑（MTT）比色法和细胞凋亡实验分别评估细胞增殖能力和细胞凋亡。采用荧光素酶报告基因实验验证调控机制。
甲状腺癌组织中 miR-524 的表达降低（p<0.05）。上调 miR-524 的表达抑制了甲状腺癌细胞的增殖能力并促进了细胞凋亡。SPAG9 是 miR-524 的靶基因，且受 miR-524 的反向调控。
miR-524 通过靶向 SPAG9 抑制甲状腺癌细胞增殖并诱导细胞凋亡。
