miR-524 通过靶向 SPAG9 抑制甲状腺癌细胞增殖并诱导细胞凋亡。
MiR-524 inhibits cell proliferation and induces cell apoptosis in thyroid cancer via targeting SPAG9.
机构信息
Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital, Beijing, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):3812-3818. doi: 10.26355/eurrev_201806_15265.
OBJECTIVE
To investigate the effect of miR-524 on the proliferation of thyroid cancer and its underlying mechanism.
PATIENTS AND METHODS
MiR-524 expression levels in thyroid cancer samples and para-cancer tissues were tested by quantitative Real-time polymerase chain reaction (qRT-PCR). Cells proliferative ability and apoptosis were evaluated through methyl thiazolyl tetrazolium (MTT) and apoptosis assays, respectively. Luciferase reporter assay was used to confirm the regulatory mechanism.
RESULTS
MiR-524 expression was reduced in thyroid cancer specimen (p<0.05). Up-regulated miR-524 expression inhibited the proliferative ability and enhanced cell apoptosis of thyroid cancer cells. SPAG9 was a target gene of miR-524, and was reversely regulated by miR-524.
CONCLUSIONS
MiR-524 represses thyroid cancer cell proliferation and induces cell apoptosis via targeting SPAG9.
目的
探讨 miR-524 对甲状腺癌细胞增殖的影响及其作用机制。
患者与方法
采用实时定量聚合酶链反应(qRT-PCR)检测甲状腺癌组织及癌旁组织中 miR-524 的表达水平。通过甲基噻唑基四唑（MTT）比色法和细胞凋亡实验分别评估细胞增殖能力和细胞凋亡。采用荧光素酶报告基因实验验证调控机制。
结果
甲状腺癌组织中 miR-524 的表达降低（p<0.05）。上调 miR-524 的表达抑制了甲状腺癌细胞的增殖能力并促进了细胞凋亡。SPAG9 是 miR-524 的靶基因，且受 miR-524 的反向调控。
结论
miR-524 通过靶向 SPAG9 抑制甲状腺癌细胞增殖并诱导细胞凋亡。
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