Department of Neurological Surgery, Peking University Ninth School of Clinical Medicine, Beijing, China.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):3847-3854. doi: 10.26355/eurrev_201806_15269.
miRNAs have been confirmed to be related to cell proliferation and apoptosis. In this study, we detected the potential effect of miR-448 on glioma cell proliferation and apoptosis.
miR-448 and CTTN expression levels were detected in glioma cell lines with quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cells were transfected with miR-448 mimics and inhibitor by using lipofectamine 2000 respectively. The proliferative ability of transfected cells was detected via methyl thiazolyl tetrazolium (MTT) and cell counting kit-8 (CCK8) assays. Cell apoptosis and cell-cycle were tested using flow cytometry. The regulatory correlation between miR-448 and CTTN was explored by bioinformatics analysis and luciferase reporter assay.
Lower expression of miR-448 and higher level of CTTN were detected in glioma cells. MiR-448 could regulate cell proliferation, cell apoptosis, and cell cycle. CTTN was negatively regulated by miR-448.
miR-448 downregulates CTTN to inhibit cell proliferation and promote apoptosis in glioma, which indicates a potential therapeutic target of glioma.
miRNA 已被证实与细胞增殖和凋亡有关。本研究旨在检测 miR-448 对神经胶质瘤细胞增殖和凋亡的潜在影响。
采用实时定量逆转录聚合酶链反应（qRT-PCR）检测神经胶质瘤细胞系中 miR-448 和 CTTN 的表达水平。分别用脂质体 2000 将 miR-448 模拟物和抑制剂转染细胞。通过甲基噻唑基四唑（MTT）和细胞计数试剂盒-8（CCK8）检测转染细胞的增殖能力。采用流式细胞术检测细胞凋亡和细胞周期。通过生物信息学分析和荧光素酶报告基因检测探索 miR-448 和 CTTN 之间的调控关系。
神经胶质瘤细胞中 miR-448 表达降低，CTTN 水平升高。miR-448 可调节细胞增殖、细胞凋亡和细胞周期。CTTN 受 miR-448 负调控。
miR-448 通过下调 CTTN 抑制神经胶质瘤细胞增殖并促进其凋亡，提示其可能成为神经胶质瘤的潜在治疗靶点。
