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A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP.

作者信息

Bullerdiek J, Dittmer J, Faehre A, Bartnitzke S, Kasche V, Schloot W

出版信息

Clin Genet. 1985 Aug;28(2):173-6. doi: 10.1111/j.1399-0004.1985.tb00379.x.

DOI:10.1111/j.1399-0004.1985.tb00379.x
PMID:2994919
Abstract

Here we present first results of an attempt to replace the nicking activity of DNAase I by ECO RI for in situ nick translation of human chromosomes. For the detection of nick translated sites we used biotinylated dUTP. The procedure resulted in a distinct banding pattern of the chromosomes which does not seem to correspond to that reached by any known banding technique.

摘要

相似文献

1
A new banding pattern of human chromosomes by in situ nick translation using ECO RI and biotin-dUTP.
Clin Genet. 1985 Aug;28(2):173-6. doi: 10.1111/j.1399-0004.1985.tb00379.x.
2
In situ nick translation distinguishes between C-band positive regions on mouse chromosomes.原位缺口平移可区分小鼠染色体上的C带阳性区域。
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Patterns of digestion of human chromosomes by restriction endonucleases demonstrated by in situ nick translation.通过原位缺口平移展示的限制性内切核酸酶对人类染色体的消化模式。
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Ultrastructural banding induced by DraI or HaeIII progressive digestion and in situ nick translation on human chromosomes.由DraI或HaeIII逐步消化以及人染色体原位缺口平移诱导产生的超微结构带型。
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