Sanaei M, Kavoosi F, Mansoori O
Research Center for Non-communicable Diseases, Jahrom University of Medical Sciences, Jahrom 74148-46199, Iran.
Student Research Committee, Jahrom University of Medical Sciences, Jahrom 74148-46199, Iran.
Exp Oncol. 2018 Jun;40(2):95-100.
Acetylation levels of histones are the result of the balance between histone acetyltransfrases and histone deacetylases activities, which plays an important role in chromatin remodeling and regulation of gene expression. Histone deacetylases inhibitors such as valproic acid, vorinostat have attracted interest because of their ability to induce differentiation and apoptosis of cancer cells. The current study was designed to assess the effect of valproic acid in comparison to and in combination with vorinostat on cell growth inhibition and apoptosis induction in the human colon cancer SW48 cells.
The colon cancer SW48 cells were seeded and treated with various doses of valproic acid and vorinostat and MTT assay and flow cytometric assay were done to determine cell viability and cell apoptosis, respectively.
All concentrations of both agents reduced viability significantly in a dose- and time-dependent fashion (p < 0.004). Both compounds, either single or combined agents, induced apoptosis significantly, whereas the ratio of the apoptotic cells treated with combined agents was more significant than the single.
Our findings suggest that vaproic acid and vorinostat can significantly inhibit cell growth and induce apoptosis in colon cancer SW48 cells.
组蛋白的乙酰化水平是组蛋白乙酰转移酶和组蛋白去乙酰化酶活性平衡的结果，这在染色质重塑和基因表达调控中起重要作用。诸如丙戊酸、伏立诺他等组蛋白去乙酰化酶抑制剂因其诱导癌细胞分化和凋亡的能力而受到关注。本研究旨在评估丙戊酸与伏立诺他相比以及联合使用时对人结肠癌SW48细胞生长抑制和凋亡诱导的影响。
接种结肠癌SW48细胞，并用不同剂量的丙戊酸和伏立诺他进行处理，分别采用MTT法和流式细胞术检测细胞活力和细胞凋亡情况。
两种药物的所有浓度均以剂量和时间依赖性方式显著降低细胞活力（p<0.004）。两种化合物，无论是单一药物还是联合用药，均显著诱导凋亡，而联合用药处理的凋亡细胞比例比单一用药更显著。
我们的研究结果表明，丙戊酸和伏立诺他可显著抑制结肠癌SW48细胞的生长并诱导其凋亡。
