Department of Medical Biology, Ege University Medical School, Izmir, Turkey.
Mol Biol Rep. 2021 Aug;48(8):6085-6091. doi: 10.1007/s11033-021-06616-2. Epub 2021 Aug 10.
Thyroid cancer is the most common malignant tumor of the endocrine system seen in the thyroid gland. More than 90% of thyroid cancers comprise papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC). Although anaplastic thyroid carcinoma (ATC) accounts for less than 2% of thyroid cancer. But patients' lifespan after diagnosis is about 6 months. Surgical interventions, radioactive iodine use, and chemotherapy are not sufficient in the treatment of ATC, so alternative therapies are needed.
The WST-1 assay test was performed to evaluate the anti-proliferative effects of Valproic acid (VPA). Also, the effect of VPA on miRNAs affecting histone deacetylase was determined by Quantitative RT-PCR. In the SW1736 cell line, IC50 dose for VPA was found 1.6 mg/ml. In our study, the level of oncogenic genes expression in cells treated with VPA, including miR-184, miR-222-5p, miR-124-3p, and miR-328-3p, decreased. Also, the expression of tumor inhibitory genes including miR-323-5p, miR-182-5p, miR-138-5p, miR-217, miR-15a-5p, miR-29b-3p, miR-324-5p and miR-101-5p increased significantly.
VPA can ad-just countless gene expression patterns, including microRNAs (miRNAs), by targeting histone deacetylase (HDAC). However, further studies are required for more accurate results.
甲状腺癌是甲状腺中最常见的内分泌系统恶性肿瘤。超过 90%的甲状腺癌包括甲状腺乳头状癌（PTC）和滤泡性甲状腺癌（FTC）。虽然间变性甲状腺癌（ATC）占甲状腺癌的比例不到 2%。但患者确诊后的寿命约为 6 个月。手术干预、放射性碘的使用和化疗在 ATC 的治疗中并不充分，因此需要替代疗法。
通过 WST-1 检测评估丙戊酸（VPA）的抗增殖作用。还通过定量 RT-PCR 确定 VPA 对影响组蛋白去乙酰化酶的 miRNA 的影响。在 SW1736 细胞系中，VPA 的 IC50 剂量为 1.6mg/ml。在我们的研究中，用 VPA 处理的细胞中致癌基因表达水平降低，包括 miR-184、miR-222-5p、miR-124-3p 和 miR-328-3p。此外，肿瘤抑制基因的表达，包括 miR-323-5p、miR-182-5p、miR-138-5p、miR-217、miR-15a-5p、miR-29b-3p、miR-324-5p 和 miR-101-5p 显著增加。
VPA 通过靶向组蛋白脱乙酰酶（HDAC）可以调节无数基因表达模式，包括 microRNAs（miRNAs）。然而，需要进一步的研究以获得更准确的结果。
