Jeong Su-Hyeon, Lee Ji-Eun, Jin Seong-Ho, Ko Youngkyung, Park Jun-Beom
J Tradit Chin Med. 2016 Dec;36(6):756-9. doi: 10.1016/s0254-6272(17)30011-0.
To examine the dose-dependent impact of Asiasari Radix (A. radix) on the cell viability, differentiation and mineralization of stem cells derived from gingiva.
Stem cells that were derived from gingiva were grown in the presence of A. radix at final concentrations that ranged from 0.001 to 10 μg/mL. The morphology of the cells was viewed under an inverted microscope and the analysis of cell proliferation was performed by using Cell Counting Kit-8 (CCK-8) on day 1. The alkaline phosphatase activity test was used to assess differentiation and Alizarin red S staining was used to assess mineralization of treated cells.
The control group showed spindleshaped, fibroblast-like morphology and the shapes of the cells in 0.001, 0.01, 0.1, 1 and 10 μg/mL of A. radix were similar to that of the control group at day 1. The cultures growing in the presence of 0.001 μg/mL of A. radix at day 1 showed an increase in the CCK-8 value (P < 0.05). Cultures growing in the presence of 0.001 μg/mL of A. radix presented the highest value for alkaline phosphatase activity (P > 0.05). Mineralized extracellular deposits were observed after Alizarin Red S staining and the cultures grown in the presence of 0.001 μg/mL of A. radix showed the highest value for quantitative results for bound dye (P < 0.05).
Within the limits of this study, A. radix influenced the proliferation of stem cells derived from the gingiva and low concentrations of A. radix might enhance osteogenic differentiation of the stem cells.
研究细辛对牙龈来源干细胞的细胞活力、分化及矿化的剂量依赖性影响。
将牙龈来源的干细胞在终浓度为0.001至10μg/mL的细辛存在下培养。在倒置显微镜下观察细胞形态，并在第1天使用细胞计数试剂盒-8（CCK-8）进行细胞增殖分析。采用碱性磷酸酶活性检测评估分化情况，茜素红S染色评估处理后细胞的矿化情况。
对照组呈现纺锤形、成纤维细胞样形态，在第1天，0.001、0.01、0.1、1和10μg/mL细辛处理组细胞的形态与对照组相似。在第1天，0.001μg/mL细辛处理组的CCK-8值升高（P<0.05）。0.001μg/mL细辛处理组的碱性磷酸酶活性最高（P>0.05）。茜素红S染色后观察到矿化的细胞外沉积物，0.001μg/mL细辛处理组的结合染料定量结果最高（P<0.05）。
在本研究范围内，细辛影响牙龈来源干细胞的增殖，低浓度细辛可能增强干细胞的成骨分化。
