中药配方HPRS乙醇提取物对HCT116细胞系的作用。
Effect of ethanol extract of HPRS, a Traditional Chinese Medicine formula, on HCT116 cell Line.
作者信息
Lu Jun, Xu Jun, Shi Qin
出版信息
J Tradit Chin Med. 2016 Dec;36(6):760-7. doi: 10.1016/s0254-6272(17)30012-2.
OBJECTIVE
In this study, we investigated the effects of ethanol extract of Hupiruisu Fang (HPRS), a formula of Traditional Chinese Medicine, on HCT116 cell line.
METHODS
MKN45, A549, Hela and HCT116 cells were treated with ethanol extract of HPRS alone or the extract plus 5-Fluorouracil (5-FU) for 48 h, and then the cell viabilities were measured using CCK-8 Kit. The early apoptosis rate and total apoptosis rate in both HCT116 cells were evaluated by flow cytometry. The mRNA levels of apoptosis-related genes including caspase-3, caspase-8, Bcl2 and Bax were detected by real-time quantitative polymerase chain reaction. Lastly, the protein activities and expressions of those apoptosis related genes were observed for further verifying the pro-apoptosis of the extract of HPRS.
RESULTS
Ethanol extract of HPRS could significantly induce apoptosis in HCT116 cell line. Synergistic analysis revealed that the extract exhibited a significant effect upon 5-FU-associated cytotoxicity in the cell line.
CONCLUSION
The ethanol extract of HPRS plus 5-FU might have the potential to improve the treatment of colorectal cancer.
目的
在本研究中，我们调查了中药配方胡吡瑞素方（HPRS）乙醇提取物对HCT116细胞系的影响。
方法
将MKN45、A549、Hela和HCT116细胞单独用HPRS乙醇提取物或提取物加5-氟尿嘧啶（5-FU）处理48小时，然后使用CCK-8试剂盒测量细胞活力。通过流式细胞术评估HCT116细胞中的早期凋亡率和总凋亡率。通过实时定量聚合酶链反应检测包括caspase-3、caspase-8、Bcl2和Bax在内的凋亡相关基因的mRNA水平。最后，观察这些凋亡相关基因的蛋白活性和表达，以进一步验证HPRS提取物的促凋亡作用。
结果
HPRS乙醇提取物可显著诱导HCT116细胞系凋亡。协同分析表明，该提取物对该细胞系中5-FU相关的细胞毒性具有显著作用。
结论
HPRS乙醇提取物加5-FU可能具有改善结直肠癌治疗的潜力。
Zotero 插件