Sugimoto T, Fukase M, Tsutsumi M, Imai Y, Hishikawa R, Yoshimoto Y, Fujita T
Endocrinology. 1985 Nov;117(5):1901-5. doi: 10.1210/endo-117-5-1901.
A new perfusion system of isolated rat femora was established, and the effect of PTH or calcitonin (CT) on cAMP release from the adult bone was examined. Stimulation of cAMP release from the isolated perfused bone peaked rapidly between 5 and 10 min after human PTH (hPTH)-(1-34) or eel CT injection, respectively, and declined gradually toward the preinjection level. However CT exhibited a longer lived effect than PTH. Release of cAMP was still significantly greater than control at 60 min after a bolus injection of CT. The rate of cAMP release was directly related to the dose of PTH or CT. H2O2-oxidized PTH (biologically inactive) caused no increase in cAMP release. hPTH-(1-34) was markedly more potent than hPTH-(1-84) in stimulating cAMP release from the perfused bone on an equimolar basis. Simultaneous administration of 5 micrograms PTH and 5 micrograms CT at maximal stimulatory doses produced additive effects, indicating the presence of separate receptor sites for PTH and CT in the bone. In conclusion, this system provides us a means by which hormone actions on adult bone with integrity of the organ can be evaluated and therefore makes possible systemic investigation of the osteoblast-osteoclast function.
建立了一种新的大鼠离体股骨灌注系统，并研究了甲状旁腺激素（PTH）或降钙素（CT）对成年骨中cAMP释放的影响。分别注射人PTH（hPTH）-（1-34）或鳗鱼CT后，离体灌注骨中cAMP释放的刺激在5至10分钟内迅速达到峰值，然后逐渐下降至注射前水平。然而，CT的作用持续时间比PTH长。在一次性注射CT后60分钟，cAMP的释放仍显著高于对照组。cAMP的释放速率与PTH或CT的剂量直接相关。H2O2氧化的PTH（无生物活性）不会导致cAMP释放增加。在等摩尔基础上，hPTH-（1-34）在刺激灌注骨中cAMP释放方面比hPTH-（1-84）明显更有效。以最大刺激剂量同时给予5微克PTH和5微克CT产生相加效应，表明骨中存在PTH和CT的独立受体位点。总之，该系统为我们提供了一种手段，通过它可以评估激素对具有器官完整性的成年骨的作用，从而使对成骨细胞-破骨细胞功能的系统研究成为可能。
