环磷酸腺苷对大鼠体内甲状旁腺激素和降钙素反应的骨骼贡献。

Skeletal contribution of cyclic adenosine monophosphate in response to parathyroid hormone and calcitonin in vivo in the rat.

作者信息

Morrison N E, Ramanathan S, Suh S M

机构信息

Department of Pharmacology, University of Hawaii, Honolulu 96822.

出版信息

J Bone Miner Res. 1988 Dec;3(6):629-34. doi: 10.1002/jbmr.5650030608.

DOI:10.1002/jbmr.5650030608

Abstract

Cyclic adenosine monophosphate (cAMP) is thought to be a second messenger for the actions of both parathyroid hormone (PTH) and calcitonin (CT). We examined the release of cAMP from rat bone in vivo after administration of synthetic rat PTH-(1-34) (rPTH), synthetic human PTH-(1-34) (hPTH), or synthetic human CT (hCT). Blood from the venous effluent of the femoral bone of rats (bone blood) was drawn at 5 and 10 minutes after the administration of hormones. The cAMP content of the bone blood was then compared to the cAMP content of arterial blood. In both kidney-clamped and non-kidney-clamped rats, hCT led to a significantly greater concentration of cAMP in the bone blood than in the arterial blood. We interpret this to be due to bone production and release of cAMP. Neither hPTH nor rPTH produced a significantly greater amount of cAMP in the bone blood than in arterial blood. These data do not preclude the possibility that there was a production of cAMP within the bone tissue itself after PTH but suggest that there was no release of cAMP from the bone into the bone blood.

摘要

环磷酸腺苷（cAMP）被认为是甲状旁腺激素（PTH）和降钙素（CT）发挥作用的第二信使。我们在给大鼠注射合成大鼠PTH-（1-34）（rPTH）、合成人PTH-（1-34）（hPTH）或合成人CT（hCT）后，检测了大鼠体内骨组织中cAMP的释放情况。在注射激素后5分钟和10分钟，采集大鼠股骨静脉流出的血液（骨血）。然后将骨血中的cAMP含量与动脉血中的cAMP含量进行比较。在肾钳夹和非肾钳夹的大鼠中，hCT导致骨血中cAMP的浓度显著高于动脉血中的浓度。我们认为这是由于骨组织产生并释放了cAMP。hPTH和rPTH均未使骨血中的cAMP含量显著高于动脉血中的含量。这些数据并不排除PTH作用后骨组织自身产生cAMP的可能性，但表明没有cAMP从骨组织释放到骨血中。