Peng Yun-Xia, Zhang Dong-Jia, Wu Wei-Guo, Mi Yong-Wei, Lin Hai-Ming
Institute of Chinese Herbal Medicines, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.
Gansu Provincial Engineering Laboratory for Genetic Improvement and Quality Control of Chinese Herbal Medicine, Lanzhou 730070, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(12):2489-2494. doi: 10.19540/j.cnki.cjcmm.20180329.008.
With Bupleurum smithii var. parvifolium and B. scorzonerifolium as test objects, in order to provide a theoretical basis for the introduction and domestication of B. smithii var. parvifolium, the growth and development dynamics of seedlings, biomass accumulation, the content of malonaldehyde(MDA), the activity of antioxidase such as SOD, POD, CAT and APX between them were comparatively analyzed by direct sowing culture in the open field. The results indicated that the morphological index and the biomass accumulation of B. smithii var. parvifolium such as root diameter, root length, plant height and leaf number were inferior to B. scorzonerifolium, the antioxidase SOD and POD activity of B. smithii var. parvifolium was significantly inferior to B. scorzonerifolium (<0.05), the antioxidase CAT and APX activity of B. smithii var. parvifolium was inferior to B. scorzonerifolium but the difference wasn't significant, while MDA content was superior to B. scorzonerifolium(<0.05). Thus, compared with cultivated B. scorzoneri folium, the plant growth velocity of wild B. smithii var. parvifolium was relatively slower and its resistance was relatively weaker after introduction and domestication.
以小叶柴胡和狭叶柴胡为试验对象，为小叶柴胡的引种驯化提供理论依据，通过田间直播栽培，对二者幼苗的生长发育动态、生物量积累、丙二醛（MDA）含量以及超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）等抗氧化酶活性进行了比较分析。结果表明，小叶柴胡的形态指标和生物量积累，如根直径、根长度、株高和叶片数等均低于狭叶柴胡；小叶柴胡的抗氧化酶SOD和POD活性显著低于狭叶柴胡（<0.05），抗氧化酶CAT和APX活性低于狭叶柴胡但差异不显著，而MDA含量高于狭叶柴胡（<0.05）。因此，与栽培狭叶柴胡相比，野生小叶柴胡引种驯化后植株生长速度相对较慢，抗性相对较弱。
