Liu Lian, Yu Hong-Li, Wang Kui-Long, Wu Hao, Wang Wei, Zhang Yuan-Bin, Yu Sheng, Yin Hou-Qing
Pharmacology College of Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(12):2516-2521. doi: 10.19540/j.cnki.cjcmm.2018.0080.
To investigate the toxicity changes of Euphorbiae Ebracteolatae Radix (EER) before and after vinegar processing, toxic diterpenoids were concentrated with chloroform as extraction solvent from EER. Then the residue was extracted for non-chloroform extract with 95% ethanol and water after extraction with chloroform. The chloroform extraction of vinegar processed EER was prepared with the same method. The mice received the drug by oral administration. Moisture content in mice feces, duodenum and colon tissue, aquaporin AQP1, AQP3, AQP4 protein expression levels were assayed as the indexes to investigate the toxicity variation of chloroform fraction, non-chloroform fraction, as well as intestinal tract toxicity before and after vinegar processing of EER. The results showed that the chloroform fraction extracted from EER could significantly increase the moisture content in mice feces, duodenum and colon, and decrease AQP1 protein expression level, increase AQP3 and AQP4 protein expression levels in the colon. The intestinal toxicity of the chloroform extract was significantly higher than that of non-chloroform extract. The moisture content in mice feces, duodenum and colon was significantly decreased, and the AQPs protein expression tended to be normal in the colon after vinegar processing. The results showed that the chloroform fraction extracted from EER could lead to diarrhea, intestinal edema, and the intestinal toxicity action was associated with interfering AQPs protein expression and promoting intestinal fluid transport disorder in mice. Vinegar-processing could reduce intestinal toxicity of EER, so vinegar processing was considered to be the scientific processing method of EER.
为研究醋制前后狼毒大戟根（EER）的毒性变化，以氯仿为提取溶剂从EER中富集毒性二萜类成分。然后用氯仿提取后的残渣再用95%乙醇和水提取非氯仿提取物。醋制EER的氯仿提取物也采用相同方法制备。小鼠经口给药。以小鼠粪便、十二指肠和结肠组织中的水分含量、水通道蛋白AQP1、AQP3、AQP4蛋白表达水平为指标，研究EER醋制前后氯仿部位、非氯仿部位的毒性变化以及肠道毒性。结果表明，从EER中提取的氯仿部位可显著增加小鼠粪便、十二指肠和结肠中的水分含量，降低AQP1蛋白表达水平，增加结肠中AQP3和AQP4蛋白表达水平。氯仿提取物的肠道毒性显著高于非氯仿提取物。醋制后小鼠粪便、十二指肠和结肠中的水分含量显著降低，结肠中AQPs蛋白表达趋于正常。结果表明，从EER中提取的氯仿部位可导致腹泻、肠道水肿，其肠道毒性作用与干扰小鼠AQPs蛋白表达、促进肠道液体转运紊乱有关。醋制可降低EER的肠道毒性，因此醋制被认为是EER的科学炮制方法。
