[醋制前后商陆正丁醇部位的肠道毒性]

[Intestinal toxicity of n-BuOH fraction from Phytolacca Radix before and after being processed with vinegar].

作者信息

Zhang Cheng-Chao, Yu Hong-Li, Wu Hao, Pan Yao-Zong, Chen Ye-Qing, Wang Kui-Long, Jin Yang-Ping, Wang Wei

机构信息

School of Pharmacy of Nangjing University of Chinese Medicine, Nangjing 210046, China.

Jiangsu Key Laboratory of Chinese Medicine Processing, Nangjing 210046, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2016 Jan;41(2):216-219. doi: 10.4268/cjcmm20160211.

DOI:10.4268/cjcmm20160211

PMID:28861966

Abstract

To research the intestinal toxicity of n-BuOH fraction in Phytolacca Radix before and after being processed with vinegar. Toxic n-BuOH fractions were separated from Phytolacca Radix. In the animal model, the level of intestinal edema, water content of intestine and stool, IC₅₀ values of HT-29 and IEC-6 were detected with MTT method to compare the changes in toxicity of n-BuOH fractions from Phytolacca Radix before and after being processed with vinegar. n-BuOH fractions of Phytolacca Radix could cause intestinal edema in mice, increase the edema of duodenum, jejunum and the water content in stool, inhibit the proliferation of HT-29 cells and IEC-6 cells, indicating its intestinal toxicity, with HT-29 IC₅₀ at 14.59 mg•L⁻¹ and IEC-6 IC₅₀ at 43.77 mg•L⁻¹. After being processed with vinegar, the level of intestinal edema, edema of duodenum and jejunum and the water content in stool and inhibition ratio of cells line were reduced, with HT-29 IC₅₀ at 58.51 mg•L⁻¹ and IEC-6 IC₅₀ at 84.37 mg•L⁻¹. After being processed with vinegar, the toxicity of n-BuOH fractions from Phytolacca Radix decreased obviously.

摘要

研究商陆正丁醇部位醋制前后的肠道毒性。从商陆中分离出有毒的正丁醇部位。在动物模型中，采用MTT法检测肠道水肿程度、肠组织含水量及粪便含水量、HT-29和IEC-6细胞的IC₅₀值，比较商陆正丁醇部位醋制前后毒性的变化。商陆正丁醇部位可导致小鼠肠道水肿，增加十二指肠、空肠水肿及粪便含水量，抑制HT-29细胞和IEC-6细胞增殖，表明其具有肠道毒性，HT-29细胞IC₅₀为14.59 mg•L⁻¹，IEC-6细胞IC₅₀为43.77 mg•L⁻¹。醋制后，肠道水肿程度、十二指肠和空肠水肿及粪便含水量和细胞系抑制率降低，HT-29细胞IC₅₀为58.51 mg•L⁻¹，IEC-6细胞IC₅₀为84.37 mg•L⁻¹。醋制后，商陆正丁醇部位的毒性明显降低。