Li Shu-Jiao, Wang Yu-Qing
Center of Scientific Research, Nanyang Medical College, Nanyang 473061, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(12):2570-2574. doi: 10.19540/j.cnki.cjcmm.20180302.001.
The present research aimed to establish an associated two-dimensional fingerprint of Huanglian between characteristic chemical composition and antioxidant activity, which was applied to on-line screen the active constituents. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in Huanglian. Fourteen compositions were discovered, and eight of them displayed antioxidant activity. The antioxidant activity of different ingredients was evaluated by antioxidant efficiency. The data showed that 2, 3, 4-trihydroxy phenylpropionic acid, chlorogenic acid, ferulic acid, cularine, 3-O-feruloylquinic acid and feruloyltyramine showed stronger antioxidant activity than that of alkaloids. These experimental data can provide data support for the basic research of the antioxidant ingredients of Huanglian.
本研究旨在建立黄连特征化学成分与抗氧化活性的二维关联指纹图谱，用于在线筛选活性成分。本研究采用HPLC-ABTS-DAD-Q-TOF/MS法，该方法能同时鉴定单个成分并快速筛选抗氧化化合物，用于筛选和鉴定黄连中的抗氧化成分。共发现14种成分，其中8种具有抗氧化活性。通过抗氧化效率评估不同成分的抗氧化活性。数据表明，2,3,4-三羟基苯丙酸、绿原酸、阿魏酸、黄连碱、3-O-阿魏酰奎尼酸和阿魏酰酪胺的抗氧化活性强于生物碱。这些实验数据可为黄连抗氧化成分的基础研究提供数据支持。
