School of Pharmaceutical Sciences, Peking University Health Science Center, 38 Xueyuan Road, Haidian District, Beijing 100083, PR China.
J Agric Food Chem. 2012 May 30;60(21):5407-13. doi: 10.1021/jf300938e. Epub 2012 May 18.
Flos Chrysanthemi (Gongju, GJ) is used to prepare a herbal tea that is commonly consumed as a health beverage in Asia and is believed to contain abundant beneficial antioxidants. To rapidly identify the chemical constituents and to obtain the profile related to antioxidant activity, an online analytical method combining high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS(n)) and postcolumn derivatization (PCD) has been applied for a precise and thorough identification of the chemical constituents. Meanwhile, the antioxidant profile has also been characterized by directly measuring the scavenging activity of each compound for the free radical produced by DPPH. As a result, 13 compounds have been identified in GJ, 7 of which account for its antioxidant activity. The established LC-MS(n)-PCD system has proved to offer a useful strategy for correlating the chemical profile with the bioactivities of the components without their isolation and purification, and may be used for multicomponent analysis of active substances in other foods and herbs.
贡菊（Gongju，GJ）被用来制作一种草本茶，在亚洲被普遍作为健康饮品饮用，被认为含有丰富的有益抗氧化剂。为了快速鉴定化学成分并获得与抗氧化活性相关的特征，应用了一种在线分析方法，将高效液相色谱-二极管阵列检测器-电喷雾电离-离子阱飞行时间质谱（HPLC-DAD-ESI-IT-TOF-MS(n)）与柱后衍生化（PCD）相结合，以精确、全面地鉴定化学成分。同时，还通过直接测量各化合物对 DPPH 自由基的清除活性来表征抗氧化特征。结果，在 GJ 中鉴定出 13 种化合物，其中 7 种化合物具有抗氧化活性。所建立的 LC-MS(n)-PCD 系统已被证明提供了一种有用的策略，可在不进行分离和纯化的情况下将化学成分与生物活性相关联，并且可用于其他食品和草药中活性物质的多组分分析。
