Wang Yu-Qing, Li Shu-Jiao, Zhuang Guo, Geng Rong-Hui, Jiang Xu
The center for Scientific Research, Nanyang Medical College, Nanyang, China.
Biomed Chromatogr. 2017 Nov;31(11). doi: 10.1002/bmc.4002. Epub 2017 Jun 20.
Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown that XXT has therapeutic effects on patients with arteriosclerosis owing to its antioxidant activity. However, there is little information about the relationship between the chemical composition of XXT and its antioxidant activity. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in XXT. The 15 compounds identified were gluco-syringic acid, adenine, gallic acid, biflorin, cularine, 6-C-arabinose-8-C-glucose-chrysin, 6-C-glucose-8-C-arabinose-chrysin, baicalin, rhein-8-O-β-d-glucopyranoside, coptisine, epiberberine, jatrorrhizine, norwogonin, 5,7,2'-trihydroxy-6- methoxyflavone and baicalein. In addition, the data showed that the antioxidant activity of peaks 4, 6, and 11 was lower in XXT than in its constituent herbs, while the activity of peaks 1, 2, 3, 5, 7, 8, 10, 12, 13, 14 and 15 was higher in XXT. Compound 5 had the strongest antioxidant activity in XXT, while compound 1 showed the strongest antioxidant activity among its constituent herb. The differences between antioxidant activities of major components of XXT and those of its constituent herbs might be due to the interaction of crude drugs that changes the solubility of active components during the decoction process. The results show that the HPLC-ABTS-DAD-Q-TOF/MS method can successfully combine on-line mass spectrometry with activity detection system. It is a useful tool for the rapid detection and identification of antioxidants, and for quantitative analysis of individual antioxidants in complex mixtures such as plant extracts. Furthermore, this method does not require extensive extract purification and fraction collection.
泻心汤（XXT）是一种已在中医诊所使用了1800多年的传统中药。许多研究表明，XXT因其抗氧化活性对动脉硬化患者具有治疗作用。然而，关于XXT的化学成分与其抗氧化活性之间的关系，目前所知甚少。在本研究中，采用能同时鉴定单个成分并快速筛选抗氧化化合物的HPLC-ABTS-DAD-Q-TOF/MS方法，对XXT中的抗氧化成分进行筛选和鉴定。鉴定出的15种化合物为葡萄糖基丁香酸、腺嘌呤、没食子酸、双花黄素、库拉灵、6-C-阿拉伯糖-8-C-葡萄糖-白杨素、6-C-葡萄糖-8-C-阿拉伯糖-白杨素、黄芩苷、大黄素-8-O-β-D-葡萄糖苷、黄连碱、表小檗碱、药根碱、去甲吴茱萸苷元、5,7,2'-三羟基-6-甲氧基黄酮和黄芩素。此外，数据显示，XXT中峰4、6和11的抗氧化活性低于其组成草药，而峰1、2、3、5、7、8、10、12、13、14和15的活性在XXT中更高。化合物5在XXT中具有最强的抗氧化活性，而化合物1在其组成草药中表现出最强的抗氧化活性。XXT主要成分与其组成草药抗氧化活性的差异可能是由于生药之间的相互作用，在煎煮过程中改变了活性成分的溶解度。结果表明，HPLC-ABTS-DAD-Q-TOF/MS方法能够成功地将在线质谱与活性检测系统相结合。它是快速检测和鉴定抗氧化剂以及对植物提取物等复杂混合物中的单个抗氧化剂进行定量分析的有用工具。此外，该方法不需要大量的提取物纯化和馏分收集。
