利用光学显微镜放射自显影术对兔耳中β-肾上腺素能受体进行定位

Localization of beta-adrenoceptors in the rabbit ear by light microscopic autoradiography.

作者信息

Johnston H G, Summers R J

出版信息

Eur J Pharmacol. 1985 Sep 10;115(1):97-101. doi: 10.1016/0014-2999(85)90589-8.

Abstract

The beta-adrenoceptor antagonist [125I]cyanopindolol (CYP) was used to localize beta-adrenoceptors in sections of rabbit ear. Biochemical studies demonstrated that the binding was stereoselective, and that the beta-adrenoceptors are predominantly of the beta 2-subtype. Autoradiographic studies using 3H-Ultrofilm or nuclear emulsion coated coverslips showed high concentrations of beta 2-adrenoceptors present in the central ear artery, hyaline cartilage, nerve trunks, epithelium and sebaceous glands.

摘要

β-肾上腺素能受体拮抗剂[125I]氰吲哚洛尔（CYP）被用于定位兔耳切片中的β-肾上腺素能受体。生化研究表明，这种结合具有立体选择性，且β-肾上腺素能受体主要为β2亚型。使用3H-Ultrofilm或涂有核乳胶的盖玻片进行的放射自显影研究显示，中耳动脉、透明软骨、神经干、上皮和皮脂腺中存在高浓度的β2-肾上腺素能受体。