Loesberg C, van Wijk R, Zandbergen J, van Aken W G, van Mourik J A, de Groot P G
Exp Cell Res. 1985 Sep;160(1):117-25. doi: 10.1016/0014-4827(85)90241-1.
We examined the influence of prostaglandins on the initiation of proliferation of growth-arrested human adult aortic and fetal smooth muscle cells. Prostaglandins of the E series (25 nM) exerted a significant (p less than or equal to 0.05) inhibitory effect on DNA synthesis. Inhibition was observed when PGE1 was added in the G1 phase of the cell cycle. PGE1 had no effect when added once DNA synthesis had started. Thus prostaglandins of the E series may inhibit the responsiveness of smooth muscle cells to the mitogenic action of critical growth factors, such as PGDF. This inhibitory response is cell-cycle dependent. Once smooth muscle cells have entered S phase, PGE1 is no longer effective. Our data also suggest that cAMP is involved in the PGE1-induced growth inhibition, since concomitant with PGE1 addition, cAMP levels rose rapidly; addition of the cAMP analogue db-cAMP resulted in a cell-cycle-dependent inhibition pattern comparable to that observed with PGE1.
我们研究了前列腺素对生长停滞的成人主动脉平滑肌细胞和胎儿平滑肌细胞增殖起始的影响。E系列前列腺素（25 nM）对DNA合成具有显著（p≤0.05）抑制作用。当在细胞周期的G1期加入PGE1时观察到抑制作用。一旦DNA合成开始后加入PGE1则无作用。因此，E系列前列腺素可能抑制平滑肌细胞对关键生长因子（如血小板衍生生长因子）的促有丝分裂作用的反应性。这种抑制反应是细胞周期依赖性的。一旦平滑肌细胞进入S期，PGE1就不再有效。我们的数据还表明，cAMP参与了PGE1诱导的生长抑制，因为与加入PGE1同时，cAMP水平迅速升高；加入cAMP类似物双丁酰cAMP导致了与PGE1观察到的类似的细胞周期依赖性抑制模式。
