Nilsson J, Olsson A G
Atherosclerosis. 1984 Oct;53(1):77-82. doi: 10.1016/0021-9150(84)90107-2.
The effect of prostaglandins (PG) on initiation of DNA synthesis in arterial smooth muscle cells (SMC) stimulated with platelet-derived growth factor (PDGF) was examined. A concentration of 10 ng/ml PGE1 inhibited DNA synthesis, measured as autoradiographically labeled nuclei, by about 70%. Similar results were obtained with PGE2 and PGD2 but at concentrations 10-20 times higher, whereas PGF2 alpha lacked effect. The inhibitory action of the prostaglandins was restricted to the first 6 h of the lag phase. Treatment with PGE1 also raised the intracellular concentration of cyclic AMP, indicating that the inhibition may be mediated via changes in the levels of cyclic nucleotides.
研究了前列腺素（PG）对血小板衍生生长因子（PDGF）刺激的动脉平滑肌细胞（SMC）中DNA合成起始的影响。浓度为10 ng/ml的前列腺素E1（PGE1）抑制DNA合成，通过放射自显影标记的细胞核测量，抑制率约为70%。前列腺素E2（PGE2）和前列腺素D2（PGD2）也得到了类似的结果，但浓度要高10 - 20倍，而前列腺素F2α（PGF2α）则没有作用。前列腺素的抑制作用仅限于延迟期的前6小时。用PGE1处理也提高了细胞内环磷酸腺苷（cAMP）的浓度，表明这种抑制可能是通过环核苷酸水平的变化介导的。
