Tarakhovsky A M, Umansky V J, Shlyakhovenko V A, Balitsky K P
FEBS Lett. 1985 Sep 23;189(2):338-40. doi: 10.1016/0014-5793(85)81051-6.
Addition of NADH, but not NAD+ or NADPH, to rat liver plasma membranes resulted in the increase of their 5'-nucleotidase activity. NADH-dependent activation of 5'-nucleotidase was significantly suppressed by atebrine, an inhibitor of NADH dehydrogenase of plasma membranes, and completely abolished by 2,4-dinitrophenol (2 X 10(-4)M) and Triton X-100 (2%). Inhibitors of electron transfer in the mitochondrial respiratory chain, rotenone and potassium cyanide, failed to affect 5'-nucleotidase activity in both the presence and absence of NADH. The data obtained give reasons to suggest a redox-dependent mechanism of 5'-nucleotidase activation in rat liver plasma membranes.
向大鼠肝细胞膜中添加NADH可使其5'-核苷酸酶活性增加，而添加NAD⁺或NADPH则无此作用。血浆膜NADH脱氢酶抑制剂阿的平可显著抑制NADH依赖的5'-核苷酸酶激活，而2,4-二硝基苯酚（2×10⁻⁴M）和 Triton X-100（2%）可使其完全丧失活性。线粒体呼吸链电子传递抑制剂鱼藤酮和氰化钾在有或无NADH的情况下均未能影响5'-核苷酸酶活性。所获数据表明，大鼠肝细胞膜中5'-核苷酸酶激活存在氧化还原依赖机制。
