Han Shuai-Shuai, Jiao Qian, Bi Ming-Xia, Du Xi-Xun, Jiang Hong
Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Medical College of Qingdao University, Qingdao 266071, China.
Ann Transl Med. 2018 May;6(10):170. doi: 10.21037/atm.2018.04.24.
SUR1, one of the subunits of ATP-sensitive potassium (K) channels, was found to be highly expressed in mRNA levels in the substantia nigra (SN) of Parkinson's disease (PD) brains. Though the mechanism of the selective dopamine (DA) neurons death is still unknown, some studies have demonstrated that selective activation of the K channels in the SN might be associated with the degeneration of DA neurons. The objective of our study is to examine the expressions of K channel subunits in dopaminergic cells with alpha-synuclein (α-Syn) transfection.
In this study, we detected the K channel subunits mRNA levels in MES23.5 cells by real-time quantitative PCR after the cells transfected with α-Syn.
Our results showed that the mRNA levels of SUR1 subunit were markedly increased by 35% in WT α-Syn overexpression cells and by 31% in A53T α-Syn overexpression cells, respectively. However, the mRNA levels of SUR2B and Kir6.2 subunit have no obviously differences from the controls.
We showed that the mRNA levels of SUR1 but not SUR2B or Kir6.2 were selectively upregulated in MES23.5 cells over-expressed with α-Syn. The findings demonstrated that the SUR1 overexpressed might be involved in the process of PD.
ATP敏感性钾（K）通道亚基之一的SUR1，被发现在帕金森病（PD）脑黑质（SN）中的mRNA水平高表达。尽管选择性多巴胺（DA）神经元死亡的机制仍不清楚，但一些研究表明，SN中K通道的选择性激活可能与DA神经元的退化有关。我们研究的目的是检测α-突触核蛋白（α-Syn）转染的多巴胺能细胞中K通道亚基的表达。
在本研究中，我们在MES23.5细胞转染α-Syn后，通过实时定量PCR检测K通道亚基的mRNA水平。
我们的结果显示，野生型α-Syn过表达细胞中SUR1亚基的mRNA水平分别显著增加35%，A53T α-Syn过表达细胞中增加31%。然而，SUR2B和Kir6.2亚基的mRNA水平与对照组无明显差异。
我们表明，在α-Syn过表达的MES23.5细胞中，SUR1的mRNA水平选择性上调，而SUR2B或Kir6.2则没有。这些发现表明，SUR1过表达可能参与了PD的发病过程。
