Reproductive and Genetic Center of Integrated Traditional and Western Medicine, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.
Department of Gynecology and Obstetrics of Traditional Chinese Medicine, The First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
Biomed Res Int. 2018 May 22;2018:1780391. doi: 10.1155/2018/1780391. eCollection 2018.
We reveal the relationship between progesterone level in follicular fluid and oocyte quality based on sequential window acquisition of all theoretical fragment-ion spectra (SWATH™), a powerful high-resolution mass spectrometric data independent acquisition technique.
Follicular fluid samples were collected from 22 subjects (the level of progesterone > 1.5 ng/mL) of progesterone group, as well as from 22 subjects (the level of progesterone < 1.5 ng/mL) of control group, and analyzed using UPLC-Q-TOF. All methods were performed in accordance with ISO 9001:2008. Novel SWATH acquisition mode on an ultra-high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (with resolving power 20,000-40,000) was investigated for the analysis of human follicular fluid. The principal component variable grouping detects intersample variable correlation and groups variables with similar profiles which simplifies interpretation and highlights related ions and fragments. It can also extract product ion spectra from the data collected by fragmenting a wide precursor ion window.
Follicular fluid from the two groups differed with respect to five metabolites. Follicular fluid from the progesterone group contained elevated levels of 8-hydroxyguanosine and 4-hydroxynonenal and reduced levels of ATP, estradiol, and L-carnitine. The increased progesterone level on the day of HCG injection could negatively impact oocyte quality, thus reducing the pregnancy rate of IVF patients.
基于序贯窗口采集所有理论片段离子谱（SWATH ™），一种强大的高分辨率质谱数据非依赖性采集技术，揭示卵泡液中孕酮水平与卵母细胞质量之间的关系。
收集 22 名孕酮组（孕酮水平> 1.5ng/mL）和 22 名对照组（孕酮水平< 1.5ng/mL）受试者的卵泡液样本，采用 UPLC-Q-TOF 进行分析。所有方法均符合 ISO 9001:2008 标准。研究了在超高效液相色谱-混合三重四极杆飞行时间质谱联用仪（分辨率 20000-40000）上的新型 SWATH 采集模式，用于分析人卵泡液。主成分变量分组检测样本间变量相关性，并对具有相似图谱的变量进行分组，从而简化解释并突出相关离子和片段。它还可以从宽前体离子窗口碎裂所采集的数据中提取产物离子谱。
两组卵泡液在 5 种代谢物上存在差异。孕酮组卵泡液中 8-羟基鸟嘌呤和 4-羟基壬烯醛水平升高，而 ATP、雌二醇和 L-肉碱水平降低。HCG 注射日孕酮水平升高可能会对卵母细胞质量产生负面影响，从而降低 IVF 患者的妊娠率。
