Maternity and Child Health Care of ZaoZhuang, ZaoZhuang, 277100, Shandong, China.
Traditional Chinese Medicine History and Literature, Institute for Literature and Culture of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.
Reprod Biol Endocrinol. 2020 May 12;18(1):42. doi: 10.1186/s12958-020-00599-8.
Follicular fluid is an important external environment for the growth and development of oocytes. A thorough identification of specific components in follicular fluid can better the existing understand of intracellular signal transduction and reveal potential biomarkers of oocyte health in women undergoing assisted reproductive therapy. To study on follicular fluid metabolomics components at different ages based on lipid metabolism, we have adopted a new method of SWATH to MRM(the sequential window acquisition of all theoretical fragment-ion spectra to multiple reaction monitor)metabolomics to provide extensive coverage and excellent quantitative data. This was done to investigate the differences in follicular fluid of patients undergoing in vitro fertilization (IVF) and embryo transfer in different age groups and to further explore the relationship between follicular fluid, age and reproductive function.
A combination of Ultra-high-performance liquid chromatography and high resolution mass spectrometry techniques were used to analyze the follicular fluid of 230 patients enrolled for the IVF cycle. The patients were of different ages grouped into two groups:the younger and older patients.The obtained multidimensional chromatographic data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The charge ratios and mass numbers enabled for the identification of different fragments in the samples. Matching information obtained through database search and the fragment information obtained by fragment ion scan structurally identified substances in the samples. This was used to determine the differential compounds.
The quality of oocytes decline with age,and the lipid composition in follicular fluid also changes,The lipid metabolism that changes with age may be related to the quality of oocytes.The main differences were in lipid metabolites. Some were up-regulated: Arachidonate, LysoPC(16:1), LysoPC(20:4) and LysoPC(20:3) while others were down-regulated: LysoPC(18:3) and LysoPC(18:1).
Metabolomic analysis of follicular fluid revealed that with the increase in age, several differential metabolites are at play. Among these metabolites, lipid metabolism undergoes significant changes that affect the development of oocytes thus causing reduced fertility in older women. These differential metabolites related to follicular development may provide possible detection and treatment targets for promoting oocyte health, and provide scientific basis for understanding the environment of oocyte development.
卵泡液是卵母细胞生长和发育的重要外部环境。对卵泡液中特定成分的全面鉴定可以更好地了解细胞内信号转导，并揭示接受辅助生殖治疗的女性卵母细胞健康的潜在生物标志物。为了研究基于脂质代谢的不同年龄卵泡液代谢组学成分，我们采用了一种新的 SWATH 至 MRM（顺序窗口采集所有理论碎片离子谱到多个反应监测）代谢组学方法，提供广泛的覆盖范围和出色的定量数据。这是为了研究不同年龄组接受体外受精 (IVF) 和胚胎移植的患者卵泡液的差异，并进一步探讨卵泡液、年龄和生殖功能之间的关系。
采用超高效液相色谱和高分辨质谱技术分析了 230 名接受 IVF 周期的患者的卵泡液。患者按年龄分为两组：年轻组和老年组。对获得的多维色谱数据进行主成分分析 (PCA) 和偏最小二乘判别分析 (PLS-DA)。通过库搜索获得的质荷比和质量数使样品中不同片段的鉴定成为可能。通过片段离子扫描获得的片段信息对样品中的物质进行结构鉴定，从而确定差异化合物。
随着年龄的增长，卵母细胞的质量下降，卵泡液中的脂质成分也发生变化。随着年龄变化的脂质代谢可能与卵母细胞的质量有关。主要差异在于脂质代谢物。一些上调：花生四烯酸、溶血磷脂酰胆碱 (16:1)、溶血磷脂酰胆碱 (20:4) 和溶血磷脂酰胆碱 (20:3)，而另一些下调：溶血磷脂酰胆碱 (18:3) 和溶血磷脂酰胆碱 (18:1)。
卵泡液代谢组学分析表明，随着年龄的增长，有几个差异代谢物在起作用。在这些代谢物中，脂质代谢发生显著变化，影响卵母细胞的发育，从而导致老年女性生育能力下降。这些与卵泡发育相关的差异代谢物可能为促进卵母细胞健康提供可能的检测和治疗靶点，并为了解卵母细胞发育环境提供科学依据。
