Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitat Autònoma de Barcelona, Avda. de Can Domènech, 08193 Cerdanyola, Spain.
Dalton Trans. 2018 Oct 16;47(40):14293-14303. doi: 10.1039/c8dt01584h.
New supramolecular systems have been prepared by mixing molybdenum organometallic metallosurfactants M(CO)5L and M(CO)4L2 {L = Ph2P(CH2)6SO3Na} with the phospholipid phosphatidylcholine. The analysis of the resulting supramolecular structures using dynamic light scattering and cryo-transmission electron microscopy has shown the formation of different aggregates depending on the metallosurfactant/phospholipid ratio, as well as a significantly different behaviour between the two studied metallosurfactants. Mixed vesicles, with properties very similar to liposomes, can be obtained with both compounds, and are called metallosomes. The formation of the mixed aggregates has also been studied by microfluidics using MeOH and EtOH as organic solvents, and it has been observed that the size of the aggregates is strongly dependent on the organic solvent used. In order to analyse the viability of these mixed systems as CO Releasing Molecules (CORMs) for biomedical applications, the CO release was studied by FT-IR spectroscopy, showing that they behave as photo-CORMs with visible and ultraviolet light. Toxicity studies of the different mixed aggregate systems have shown that metallosomes exhibit a very low toxicity, similar to liposomes that do not contain metallosurfactants, which is well below the results observed for pure metallosurfactants. Micro-FTIR microscopy using synchrotron radiation has shown the presence of metallosurfactants in cells. The results of this study show the influence of the length of the hydrocarbon chain on the properties of these mixed systems, compared with previously reported data.
已制备了新的超分子体系，方法是将钼有机金属金属表面活性剂 M(CO)5L 和 M(CO)4L2 {L = Ph2P(CH2)6SO3Na} 与磷脂酰胆碱混合。使用动态光散射和低温透射电子显微镜对所得超分子结构的分析表明，形成了不同的聚集物，这取决于金属表面活性剂/磷脂的比例，以及所研究的两种金属表面活性剂之间的行为存在显著差异。可以用这两种化合物获得具有非常类似于脂质体的性质的混合囊泡，并将其称为金属囊泡。还使用甲醇和乙醇作为有机溶剂通过微流控技术研究了混合聚集物的形成，观察到聚集物的尺寸强烈依赖于所用的有机溶剂。为了分析这些混合体系作为用于生物医学应用的 CO 释放分子（CORMs）的可行性，通过傅里叶变换红外（FT-IR）光谱研究了 CO 的释放，结果表明它们作为光 CORMs 用可见光和紫外线起作用。对不同混合聚集体系的毒性研究表明，金属囊泡表现出非常低的毒性，类似于不含金属表面活性剂的脂质体，远低于纯金属表面活性剂的观察结果。使用同步辐射的微 FT-IR 显微镜显示了金属表面活性剂在细胞中的存在。这项研究的结果表明，与以前报道的数据相比，碳氢链长度对这些混合体系的性质有影响。
