Universitat Autònoma de Barcelona, Biophysics Unit/Center for Biophysical Studies, Department of Biochemistry and Molecular Biology, Faculty of Medicine, 08193 Cerdanyola del Vallès, Spain.
Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau - Centre CERCA, Genomics of Complex Diseases, Barcelona, Spain.
Colloids Surf B Biointerfaces. 2023 Aug;228:113422. doi: 10.1016/j.colsurfb.2023.113422. Epub 2023 Jun 21.
The scientific relevance of carbon monoxide has increased since it was discovered that it is a gasotransmitter involved in several biological processes. This fact stimulated research to find a secure and targeted delivery and lead to the synthesis of CO-releasing molecules. In this paper we present a vesicular CO delivery system triggered by light composed of a synthetized metallosurfactant (TCOL10) with two long carbon chains and a molybdenum-carbonyl complex. We studied the characteristics of mixed TCOL10/phosphatidylcholine metallosomes of different sizes. Vesicles from 80 to 800 nm in diameter are mainly unilamellar, do not disaggregate upon dilution, in the dark are physically and chemically stable at 4 °C for at least one month, and exhibit a lag phase of about 4 days before they show a spontaneous CO release at 37 °C. Internalization of metallosomes by cells was studied as function of the incubation time, and vesicle concentration and size. Results show that large vesicles are more efficiently internalized than the smaller ones in terms of the percentage of cells that show TCOL10 and the amount of drug that they take up. On balance, TCOL10 metallosomes constitute a promising and viable approach for efficient delivery of CO to biological systems.
一氧化碳的科学相关性增加了，因为人们发现它是一种参与多种生物过程的气体递质。这一事实刺激了研究人员寻找安全且靶向的输送方法，并导致了 CO 释放分子的合成。在本文中，我们介绍了一种由合成的金属表面活性剂（TCOL10）和钼羰基配合物组成的光触发的囊泡 CO 输送系统。我们研究了不同大小的混合 TCOL10/磷脂酰胆碱金属囊泡的特性。直径为 80 至 800nm 的囊泡主要是单分子层的，在稀释时不会聚集，在黑暗中，在 4°C 下至少稳定一个月，在 37°C 下自发释放 CO 之前有大约 4 天的滞后期。我们研究了细胞内化金属囊泡的功能，作为孵育时间、囊泡浓度和大小的函数。结果表明，大囊泡比小囊泡更有效地内化，这体现在显示 TCOL10 的细胞百分比和它们摄取的药物量上。总的来说，TCOL10 金属囊泡是一种很有前途和可行的方法，可有效地将 CO 输送到生物系统中。
