Miller-Faurès A, Blave A, Caudron M, Sene C, Miller A O
Dev Biol Stand. 1985;60:209-18.
Applying Hydroxyurea, Nocodazole and Aphidicolin in succession to obtain parasynchronous growth, the progression of HTC and HeLa cells through the cell cycle has been monitored by laser flow cytometry. The experimental results show that HTC cells behave identically whether grown in monolayer or attached to dextran-based microbeads but that the chemical nature of the micro-support itself plays an important role especially on the speed with which the cells pass from mitosis into G1, polyacrylamide-based microbeads being superior in this respect.
依次应用羟基脲、诺考达唑和阿非迪霉素以实现细胞同步生长，通过激光流式细胞术监测了HTC和HeLa细胞在细胞周期中的进程。实验结果表明，HTC细胞无论是单层生长还是附着于基于葡聚糖的微珠上，其行为都是相同的，但微载体本身的化学性质起着重要作用，特别是在细胞从有丝分裂进入G1期的速度方面，基于聚丙烯酰胺的微珠在这方面表现更优。
