紫杉醇的体外抗肝癌活性。
Anti-hepatoma activity of taxol in vitro.
作者信息
Yuan J H, Wang X W, Luo D, Xie Y, Xie H
机构信息
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
出版信息
Acta Pharmacol Sin. 2000 May;21(5):450-4.
AIM
To investigate the in vitro anti-hepatoma activity of taxol against SMMC-7721 human hepatoma cells.
METHODS
The hepatoma cell growth was measured by MTT-microculture tetrazolium assay. Cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination.
RESULTS
Taxol inhibited the hepatoma cell growth in concentration- and time-dependent manners with IC50 of 18.96 nmol.L-1. Marked cell accumulation in G2/M phase and multinucleated cells were also observed after treatment with taxol 10 nmol.L-1. In addition, taxol at 10 nmol.L-1 could induce the apoptosis of hepatoma cells.
CONCLUSION
Taxol suppresses the growth of SMMC-7721 human hepatoma cells in vitro by causing cell-cycle arrest, aberrant mitosis, and apoptosis of the human hepatoma cells.
目的
研究紫杉醇对人肝癌SMMC - 7721细胞的体外抗肝癌活性。
方法
采用MTT微培养四唑盐法检测肝癌细胞生长情况。通过流式细胞术和显微镜检查分析细胞周期动力学和细胞凋亡情况。
结果
紫杉醇以浓度和时间依赖性方式抑制肝癌细胞生长，IC50为18.96 nmol·L-1。用10 nmol·L-1紫杉醇处理后，还观察到明显的G2/M期细胞堆积和多核细胞。此外，10 nmol·L-1的紫杉醇可诱导肝癌细胞凋亡。
结论
紫杉醇通过导致细胞周期停滞、异常有丝分裂和人肝癌细胞凋亡，在体外抑制SMMC - 7721人肝癌细胞的生长。
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