College of Science, China University of Petroleum, No. 66, West Changjiang Road, Qingdao, 266580, China.
Macromol Rapid Commun. 2018 Aug;39(15):e1800263. doi: 10.1002/marc.201800263. Epub 2018 Jun 27.
A simplified origami strategy to create large-scale complex DNA nanostructures based on the rolling circle amplification (RCA) is developed. The long repetitive block single strand DNA (ssDNA) synthesized from RCA with a few staple strands are used to fabricate DNA origami, which can be assembled into the first level assemblies of micro-scale length mono-nanoladders. Depending on base pairing among the sticky ends of short staple strands in nanoladders, the second level assemblies with large-scale and controlled structures such as tri-nanoladder, penta-nanoladder, and even nanobrocade, can be further achieved.
开发了一种基于滚环扩增（RCA）的简化折纸策略，用于创建大规模复杂 DNA 纳米结构。使用来自 RCA 的几个短链 staple strands 的长重复块单链 DNA（ssDNA）来制造 DNA 折纸，可以将其组装成微尺度长度的单纳米梯的第一级组装。取决于短链 staple strands 粘性末端之间的碱基配对，可以进一步实现具有大规模和可控结构的第二级组装，如三纳米梯、五纳米梯，甚至纳米串珠。
